Microbiology with Diseases by Taxonomy (5th Edition)
Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 20, Problem 11MC
Summary Introduction

Introduction:

Q fever is a disease found all over the world, caused by the bacterium Coxiella. It is named so because its origin was doubtful or “questionable” for many years. It is found in people who work with animals, like veterinarians and cattlemen. Q fever is of two types- Acute that lasts for about 20 days with mild symptoms, and chronic continues for months to years, leading to fatal condition like endocarditis, and inflammation of internal organs like lungs and liver. It is treated with prolonged doses of antimicrobial drugs.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

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Microbiology with Diseases by Taxonomy (5th Edition)

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