BIOCHEM. II-EBOOK ACCESS>CUSTOM<
BIOCHEM. II-EBOOK ACCESS>CUSTOM<
18th Edition
ISBN: 9781337700320
Author: GARRETT
Publisher: CENGAGE LEARNING (CUSTOM)
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Chapter 2, Problem 21P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

The enzyme alcohol dehydrogenase catalyzes the oxidation of ethyl alcohol by NAD+ to give acetaldehyde plus NADH and a proton:

CHjCH3OH + NAD+ > C’H3CHO + NADH + H+

The rate of this reaction can be measured by following the change in pH. The reaction is run in 1-mL 10 m TRIS buffer at pH 8.6. If the pH of the reaction solution falls to 8.4 after 10 minutes, what is the rate of alcohol oxidation, expressed as nanomoles of ethanol oxidized per mL per sec of reaction mixture?

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