Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Question
Chapter 2, Problem 20CQ
Summary Introduction
To calculate: The standard free energy change for the dissociation of HEPES.
Concept introduction: The standard free energy is the Gibbs free energy (G) that indicates the continuous series of reaction more appropriately than entropy. HEPES is considered as an organic chemical buffering agent that is usually used to sustain the physiological pH range 6.8-8.2.
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Solution
? Hint
Sub
bo Each pictured Lewis structure is invalid. Identify the error in each case.
O Macmillan Learning
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Answer Bank
wrong electron total
:0-
:F======F:
octet-rule violation
N
:0:
[s] mM
V (M/s)
Uninhibited
0.333
1.65 x 107
1.05 x 107
V (M/s) x 10'
Inhibitor A
V (M/s) x 107
Inhibitor B
0.794 x 107
0.40
1.86 x 107
1.21 x 107
0.893 x 107
0.50
2.13 x 107
1.43 x 107
1.02 x 107
0.666
2.49 x 107
1.74 x 107
1.19 x 107
1.0
2.99 x 107
2.22 x 107
1.43 x 107
2.0
3.72 x 107
3.08 x 107
1.79 x 107
For a Michaelis-Menten reaction, k₁-5 x 10'/M-s, k.-2 x 10%/s, and k₂-4 x 10²/s.
a)
Calculate the Ks and KM for this reaction.
b)
Does substrate binding achieve equilibrium or steady state?
Chapter 2 Solutions
Fundamentals of Biochemistry: Life at the Molecular Level
Ch. 2 - Prob. 1ECh. 2 - 2. Rank the water solubility of the following...Ch. 2 - 3. Approximately how many molecules of H2O are in...Ch. 2 - 4. An E. coli cell contains about 2.6 × 108 ions,...Ch. 2 - Prob. 5ECh. 2 - Prob. 6ECh. 2 - 7. Describe what happens when a dialysis ban...Ch. 2 - 8. A red blood cell his an internal salt...Ch. 2 - 9. Draw the structures of the conjugate bases of...Ch. 2 - Prob. 10E
Ch. 2 - 11. Indicate the ionic species of ammonia that...Ch. 2 - 12. Indicate the ionic species of phosphoric acid...Ch. 2 - 13. Calculate the pH of a 200 mL solution of pure...Ch. 2 - 14. Calculate the pH of a 1 L solution containing...Ch. 2 - 15. A solution is nude by mixing 50 mL of 2.0 M...Ch. 2 - 16. What is the pK of the weak acid HA if a...Ch. 2 - 17. (a) At any instant, how many water molecules...Ch. 2 - 18.
Would phosphoric acid or succinic acid be a...Ch. 2 - 19. Use Coulomb’s law (Equation 2-1) to explain...Ch. 2 - Prob. 20CQCh. 2 - 21. Certain C—H groups can form weak hydrogen...Ch. 2 - 22. Explain why water forms nearly spherical...Ch. 2 - Prob. 23CQCh. 2 - 24. You have a 5 ml. sample of a protein in 0.5 M...Ch. 2 - 25. Patients with kidney failure frequently...Ch. 2 - 26. The kidneys function to eliminate ammonia from...Ch. 2 - Prob. 27CQCh. 2 - Prob. 28CQCh. 2 - 29. How many grams of sodium succinate (formula...Ch. 2 - 30. Estimate the volume of a solution of 5 M NaOH...Ch. 2 - 31. You need a buffer at pH 7.5 for use in...Ch. 2 - Prob. 32CQCh. 2 - MORE TO EXPLORE Compare and contrast the...
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