(a)
To explain: Whether the phosphoric acid or succinic acid is a better buffer at pH 5.
Concept introduction: The pH of a solution is denoted as the negative logarithm of the hydrogen ion concentration of that solution. The pH in a solution decreases as H+ ion concentration increases in the solution. The pH of a neutral aqueous solution is 7, if it exceeds 7, then it is a base and if it below 7 it is an acid. A buffer is the substance that minimizes the changes in the concentration of H+ and OH-.
(b)
To explain: Whether the ammonia or piperidine is a better buffer at pH 9.
Concept introduction: The pH of a solution is denoted as the negative logarithm of the hydrogen ion concentration of that solution. The pH in a solution decreases as H+ ion concentration increases in the solution. The pH of a neutral aqueous solution is 7, if it exceeds 7, then it is a base and if it below 7, then it is an acid. A buffer is the substance that minimizes the changes in the concentration of H+ and OH-.
(c)
To explain: Whether the HEPES or Tris is a better buffer at pH 5.
Concept introduction: The pH of a solution is denoted as the negative logarithm of the hydrogen ion concentration of that solution. The pH in a solution decreases as H+ ion concentration increases in the solution. The pH of a neutral aqueous solution is 7, if it exceeds 7, then it is a base and if it below 7 it is an acid. A buffer is the substance that minimizes the changes in the concentration of H+ and OH-.
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FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
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