Human Physiology
15th Edition
ISBN: 9781259864629
Author: Fox, Stuart Ira
Publisher: Mcgraw-hill Education,
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Chapter 2, Problem 15RA
Summary Introduction
To determine:
Type of bond between the nitrogen bases in the double-stranded DNA.
Introduction:
DNA (deoxyribonucleic acid) is the genetic material of multicellular organisms and is a double stranded molecule having complementary strands to each other. DNA contains nitrogen bases, ribose sugar, and phosphate group. The nitrogen bases are attached to each other via hydrogen bonds.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 2 Solutions
Human Physiology
Ch. 2 - List the components of an atom and explain how...Ch. 2 - Describe the nature of nonpolar and polar covalent...Ch. 2 - Define the terms acidic, basic, acid, and base....Ch. 2 - Using chemical equations, explain how bicarbonate...Ch. 2 - Prob. 4CPCh. 2 - Describe the structural characteristic of all...Ch. 2 - Describe the characteristics of a lipid, and...Ch. 2 - Explain, in terms of dehydration synthesis and...Ch. 2 - Relate the functions of phospholipids to their...Ch. 2 - Write the general formula for an amino acid, and...
Ch. 2 - Describe and account for the different levels of...Ch. 2 - Describe the different categories of protein...Ch. 2 - Prob. 10aCPCh. 2 - Prob. 10bCPCh. 2 - Describe the structure of DNA, and explain the law...Ch. 2 - Which of these statements about atoms is true?...Ch. 2 - The bond between oxygen and hydrogen in a water...Ch. 2 - Which of these is a nonpolar covalent bond?...Ch. 2 - Solution A has a pH of 2, and solution B has a pH...Ch. 2 - Glucose is
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Ch. 2 - Which of these Lipids have regulatory roles in the...Ch. 2 - The tertiary structure of a protein is directly...Ch. 2 - The type of bond formed between two molecules of...Ch. 2 - The carbon-to-nitrogen bond that joins amino acids...Ch. 2 - Prob. 13RACh. 2 - Prob. 14RACh. 2 - Prob. 15RACh. 2 - Prob. 16RACh. 2 - Prob. 17RACh. 2 - Explain, in terms of dehydration synthesis and...Ch. 2 - Prob. 19RACh. 2 - Prob. 20RACh. 2 - Explain how one DNA molecule serves as a template...Ch. 2 - Prob. 22RACh. 2 - Prob. 23RACh. 2 - From the ingredients listed on a food wrapper, it...Ch. 2 - Prob. 25RACh. 2 - Prob. 26RACh. 2 - Prob. 27RACh. 2 - Prob. 28RACh. 2 - Prob. 29RACh. 2 - The molecular weight is the sum of the atomic...
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