Biological Science (7th Edition)
7th Edition
ISBN: 9780134678320
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Greg Podgorski, Emily Taylor, Jeff Carmichael
Publisher: PEARSON
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Chapter 2, Problem 14PIAT
Summary Introduction
To explain:
The mechanism by which antifreeze protein (AFP) prevents cells from freezing solid.
Introduction:
The freezing of water molecules causes the formation of four hydrogen bonds at one time. This results in the formation of the low-density ice crystals. The formation of the crystals can be prevented by the formation of the hydrogen bonds.
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a. You selectively label phospholipids with a fluorescent dye and perform the FRAP assay. You detect a single fluorescence molecule move 100 micrometre into the photobleached area in 2.5 seconds. What is the diffusivity constant for this phospholipid?
b. Increasing the ratio of unsaturated to saturated phospholipids in a biological membrane increases membrane fluidity. Describe how the FRAP photobleaching and recovery curves would differ between membranes with 63% and 41% unsaturated phospholipids.
c. Draw the FRAP photobleaching and recovery curves for your answer in b.
d. What does the “immobile fraction” in a FRAP experiment suggest (see diagram)?
Consider a mixture comprised of the proteins below:
Protein that will most strongly bind to an anion exchange column is?
Protein that will elute first in gel filtration chromatography is?
Protein that will elute last in hydrophobic interaction chromatography is?
Protein that will elute last in carbohydrate containing column is ?
Chapter 2 Solutions
Biological Science (7th Edition)
Ch. 2 - Which of the following occurs when a covalent bond...Ch. 2 - Prob. 4TYKCh. 2 - 5. Which of the following molecules would you...Ch. 2 - Locate fluorine (F) on the partial periodic table...Ch. 2 - Prob. 7TYUCh. 2 - Prob. 8TYUCh. 2 - From what you have learned about water, why do...Ch. 2 -
How do organisms survive below freezing...Ch. 2 - 12. The salty ocean has a higher level of entropy...Ch. 2 - Prob. 14PIAT
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