Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 2, Problem 10P
Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.
Calculate the pH Change in a Phosphate Buffer When Acid or Base Is Added
- If 50 mL of 0.01 M HCL is added to 100 mL of 0.05 M phosphate buffer at pH 7.2, what is the resultant pH? What are the concentrations of H2POh- and HPO42- in the final solution?
- If 50 mL of 0.01 M NaOH is added to 100 mL of 0.05 M phosphate buffer at pH 7.2, what is the resultant pH0 What are the concentrations of H2P04- and HPO42- in this final solution?
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Chapter 2 Solutions
Biochemistry
Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...
Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Prob. 14PCh. 2 - Answers to all problems are at the end of this...Ch. 2 - Prob. 16PCh. 2 - Answers to all problems are at the end of this...Ch. 2 - Prob. 18PCh. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Answers to all problems are at the end of this...Ch. 2 - Prob. 22PCh. 2 - Answers to all problems are at the end of this...
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- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Calculating [H+] from pH Calculate the following from the pH values given in Table 2.3. [H+] in vinegar [H+] in saliva [H+] in household ammonia [OH-] in milk of magnesia [OH-] in beer [H+] inside a liver cellarrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Plot the Titration Curve for Bicine and Calculate How to Prepare a pH 7.5 Bicine Buffer Solution Bicine (N, N—bis (2-hydroxyethyl) glycine) is another commonly used buffer in biochemistry labs. The structure of Bicine in its fully protonated form is shown here: Draw the titration curve for Bicine. assuming the pA'a for its free COOH group is 2.3 and the pAa for its tertiary amino group is 8.3. Draw the structure of the fully deprotonated form (completely dissociated form) of bicine. You have available a U.l Msolution of Bicine at its isoelectric point (pH|)T 0.1 M solutions of HCI and NaOH. and ample distilled water. Describe the preparation of 1 L of 0.U4 M Bicine buffer. pH 7.5. What is the concentration of the fully protonated form of Bicine in your final buffer solution?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Calculating [H] and pKd from the pH of a Solution of Weak Acid The pH of a 0.02 M solution of an acid was measured at 4.6. What is the [H+] in this solution? Calculate the acid dissociation constant Ka and pKa for this acid.arrow_forward
- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Calculating the pH of a Solution of a Weak Acid; Calculating the pH of the Solution after the Addition of Strong Base The ka for formic acid is 1.78 10-4 M. What is the pH of a 0.1 M solution of formic acid? 150 mL of 0.1 M NaOH is added to 200 mL of 0.1 M formic acid, and water is added to give a final volume of 1 L. What is the pH of the final solution?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Draw the Titration Curve for a Weak Acid and Determine its pKa from the Titration Curve When a 0.1 M solution of a weak acid was titrated with base, the following results were obtained: Plot the results of this titration and determine the pK a of the weak acid from your graph.arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Preparing a Phosphate Buffer Solution of pH 7.5 from Solutions of Na3PO4 and H3PO4 Given 0.1 M solutions of Na3PO4, and H3PO4 describe the preparation of 1 L of a phosphate buffer at a pH of 7.5. What are the molar concentrations of the ions in the final buffer solution, including Na+ and H+?arrow_forward
- Answers to all problems are at (he end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Understanding State Functions Define a slate function. Name three thermodynamic quantities that are state functions and three thatarrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Cells as Steady-State Systems Describe what is meant by the phrase "cells tire steady-state systems." (Section 1.4)arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Exploring the Michaelis-Menten Equation - II If Vmax=100mol/mLsecand Km=2mM, what is the velocity of the reaction when [S] = 20 mM?arrow_forward
- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Calculate the pH in a Muscle Cell from the HPO42-/H2PO4Ratio If the internal pH of a muscle cell is 6.8, what is the [HPO42-]/ [H2PO4-] ratio in this cell?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Polyprotic Adds: Phosphate Species Abundance at Different pHs Whatare the approximate fractional concentrations of the following phosphate species at pH values of 0,2, 4, 6, 8, 10, and 12? H3PO4 24- HPO42- PO43-arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Prepare a Buffer by Combining a Solution of Weak Acid with a Solution of the Salt of the Weak Acid Given 0.1 M solutions of acetic acid and sodium acetate, describe the preparation of 1 L of 0.1 M acetate buffer at a pH of 5.4.arrow_forward
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