Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 19.3, Problem 3LO
Summary Introduction
To determine: The difference between different types of stem cells.
Introduction: Erythropoietin is a naturally occurring glycoprotein found in the body synthesized inside the interstitial fibroblasts in the kidney. The primary function of this protein istostimulateerythropoiesis (formation of red blood cells) inside the bone marrow. The stem cell, hemocytoblast, promotes the blood to carry more oxygen.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 19 Solutions
Biology
Ch. 19.1 - Prob. 1LOCh. 19.2 - Prob. 1LOCh. 19.2 - Prob. 2LOCh. 19.2 - Distinguish differences in cell division between...Ch. 19.3 - Prob. 1LOCh. 19.3 - Prob. 2LOCh. 19.3 - Prob. 3LOCh. 19.4 - Prob. 1LOCh. 19.4 - Prob. 2LOCh. 19.5 - Prob. 1LO
Ch. 19.5 - Describe D/V axis formation in Drosophila.Ch. 19.6 - Prob. 1LOCh. 19.6 - Prob. 2LOCh. 19.6 - Prob. 3LOCh. 19.7 - Prob. 1LOCh. 19.7 - Prob. 2LOCh. 19.7 - Prob. 3LOCh. 19 - What type of cells would develop if you injected...Ch. 19 - The sheep used for the donor nucleus had a...Ch. 19 - During development, cells become a. differentiated...Ch. 19 - Prob. 2UCh. 19 - Prob. 3UCh. 19 - Prob. 4UCh. 19 - Plant meristems a. are only present during...Ch. 19 - Prob. 6UCh. 19 - The process of nuclear reprogramming a. is a...Ch. 19 - What is the common theme in cell determination by...Ch. 19 - The process of reproductive cloning a. shows that...Ch. 19 - Prob. 3ACh. 19 - Prob. 4ACh. 19 - The genes that encode the morphogen gradients in...Ch. 19 - What would be the likely result of a mutation of...Ch. 19 - MADS-box, and Hox genes are a. found only in...Ch. 19 - The fate map for C. elegans (refer to figure 19.3)...Ch. 19 - Prob. 2SCh. 19 - You have generated a set of mutant embryonic mouse...Ch. 19 - Assume you have the factors in hand necessary to...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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