Microbiology: A Systems Approach
Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 19.3, Problem 10AYP
Summary Introduction

To explain:

The difference between oral polio vaccine and inactivated polio vaccine.

Concept introduction:

Poliomyelitis is an enteroviral infection which affects the spinal cord and is characterized by neuromuscular paralysis. Poliomyelitis is caused by poliovirus belonging to family Picornaviridae and genus Enterovirus.

Summary Introduction

To explain:

Advantages and disadvantages of oral polio vaccine and inactivated polio vaccine.

Concept introduction:

Poliomyelitis is caused by poliovirus belonging to family Picornaviridae and genus Enterovirus. There are two vaccines available for poliomyelitis, namely inactivate Salk poliovirus vaccine and attenuated oral Sabin poliovirus vaccine.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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