PRESCOTT'S MICROBIO W/PROCTORIO
11th Edition
ISBN: 9781264731060
Author: WILLEY
Publisher: MCG
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Chapter 19.2, Problem 2CC
Why is it important to have a type strain for each species?
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Is it possible to obtain DNA in cloverleaf configuration? Explain.
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The following two sequences represent the same gene from two different species: Assume that all changes in the third position are synonymous and all in the first and second positions are nonsynonymous.
a) How many synonymous sites (positions) are there in the gene?
b) How many nonsynonymous sites (positions) are there in the gene?
c) How many synonymous substitutions are there between species?
d) How many nonsynonymous substitutions are there between species?
e) What is the dN/dS ratio?
f) What does the dN/dS ratio tell you about the evolution of this sequence?
Finally, It has been hypothesized that population size affects genome size. What is the proposed relationship? How can it be explained?
Chapter 19 Solutions
PRESCOTT'S MICROBIO W/PROCTORIO
Ch. 19.1 - What is a natural classification? What microbial...Ch. 19.1 - Prob. 2CCCh. 19.1 - Consider the finding that bacteria capable of...Ch. 19.2 - What is the difference between a microbial species...Ch. 19.2 - Why is it important to have a type strain for each...Ch. 19.2 - The genus Salmonella was once thought to contain...Ch. 19.3 - What are the advantages of using each major group...Ch. 19.3 - Why is rRNA so suitable for determining...Ch. 19.3 - Prob. 3CCCh. 19.4 - Could a phylotype be considered an OTU? What about...
Ch. 19.4 - List the differences between distance-based and...Ch. 19.4 - Prob. 3CCCh. 19.4 - You are building a tree based on 16S rRNA sequence...Ch. 19.4 - Is HGT involved in movement of genes in the core...Ch. 19.5 - On what evidence is this hypothesis based?Ch. 19.5 - Construct a scenario in which each of the...Ch. 19.5 - Define ecotype. Do you think it is necessary to...Ch. 19.5 - What is the difference between the core genome and...Ch. 19.5 - Of the following genes, which do you think are...Ch. 19.5 - Prob. 4CCCh. 19.6 - Prob. 1CCCh. 19.6 - Describe two different situations in which it...Ch. 19 - Prob. 1RCCh. 19 - Prob. 2RCCh. 19 - Prob. 3RCCh. 19 - Prob. 4RCCh. 19 - Prob. 5RCCh. 19 - Prob. 6RCCh. 19 - Consider the fact that the use of 16S rRNA...Ch. 19 - You have recently established a pure culture of a...Ch. 19 - Prob. 3ALCh. 19 - Prob. 4ALCh. 19 - Prob. 5ALCh. 19 - Prob. 6ALCh. 19 - Prob. 7AL
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- In a genomic analysis looking for a specific disease gene, one candidate gene was found to have a single-base-pair substitution resulting in a nonsynonymous amino acid change. What would you have to check before concluding that you had identified the disease-causing gene?arrow_forwardWhich of the following pairs of sequences would be considered different alleles in DNA profiling? a) ATGAATTCGG; ATGAAATCGG b) ATGAATTCGG; TACTTACTTACT c) GAAGAAGAA; GAAGAAGAAGAA d) AATAATAATAAT; AATTAATTAATTarrow_forwardYou isolate bacteria from several different pools at the recreation center. Curious about which bacteria likely have a common ancestor with your reference pool (the Buffalo-pool), you BLAST the 16S ribosomal gene from the Buffalo pool bacteria against the 16S ribosomal genes from the other pools. The results are shown below. Which of the bacteria likely have a common ancestor with the Buffalo-pool bacteria? Use e as a cutoff for homology. Organism Dive-well bacteria Competition-pool bacterial Practice-pool bacteria Puddle bacteria Puddle bacteria Practice-pool bacteria Competition-pool bacteria Dive-well bacteria E-value 9e-175 -300 8e 4e-2 4arrow_forward
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