Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 19, Problem 6PDQ
Summary Introduction
To review:
The creation of synthetic genome by the team of J. Craig Venter Institute and demonstration of the developed synthetic genome in the conversion of recipient strain of bacteria into a different strain.
Introduction:
Synthetic genome allows the researchers to produce the genetically engineered and modified organisms. These genetically engineered organisms possess the unique characteristics and have a significant commercial value. Synthetic genomes also lead to the production of certain novel characters in the recipient cell after the transformation.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning
b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloning
Following are four processes common to most cloning experiments:
a) transforming bacteria
b) plating bacteria on selective medium
c) cutting DNA with restriction endonucleases
d) ligating DNA fragments
Place components of this list in the order in which they would most likely occur during a cloning experiment.
Which method is used to obtain mutants that grow under conditions that the wild type parent cannot grow?
a)indirect selction
b) direct selection
c) screening for possible mutagen ( carcinogens)
d) replica plating
Chapter 19 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 19 - CASE STUDY| Cancer-killing bacteria Ralph, a...Ch. 19 - CASE STUDY| Cancer-killing bacteria Ralph, a...Ch. 19 - CASE STUDY |Cancer-killing bacteria Ralph, a...Ch. 19 - HOW DO WE KNOW? In this chapter, we focused on a...Ch. 19 - Prob. 2PDQCh. 19 - Why are most recombinant human proteins produced...Ch. 19 - Prob. 4PDQCh. 19 - One of the major causes of sickness, death, and...Ch. 19 - Prob. 6PDQCh. 19 - Sequencing the human genome and the development of...
Ch. 19 - Prob. 8PDQCh. 19 - As genetic testing becomes widespread, medical...Ch. 19 - What limits the use of differences in restriction...Ch. 19 - Might it make sense someday to sequence every...Ch. 19 - What is the main purpose of genome-wide...Ch. 19 - Prob. 13PDQCh. 19 - Prob. 14PDQCh. 19 -
15. In 2013 the actress Angelina Jolie elected to...Ch. 19 - Prob. 16PDQCh. 19 - Should the FDA regulate direct-to-consumer genetic...Ch. 19 - Prob. 18PDQCh. 19 - Prob. 19PDQCh. 19 - Private companies are now offering personal DNA...Ch. 19 - Yeager, M., et al.(Nature Genetics 39: 645–649,...Ch. 19 - In March 2010 Judge R. Sweet ruled to invalidate...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forwardThe following DNA sequence is from a bacteriophage that infects a pathogenic bacterium and scientists want to know if this bacteriophage could prove to be a potential treatment against it. But first scientists need to discover if different strains of this pathogen have restriction endonucleases that it may use for its own protection. They try 3 different RE’s:a) EcoR1 b) HaeIII c) BamH1 Look up the recognition sequences for the 3 Res. Enzymes above and check whether the phage genome (a snippet of which is shown below) will or will not be ‘cut’. Tell me how their experiment worked out and what their conclusion was.G A A A A G G C C A C A A G G C C G T C G A C T T T T A A A A G G C C A C A T G C G G C T T T T C C G G T G T T C C G G C AG C T GA A A AT T T T C C G G T G T A C G CCarrow_forwardWhat is a cloning vector? Give two examples of specific DNA molecules routinely used as cloning vectors.arrow_forward
- Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which alsoserved as a model system for the study of bacteriophage morphogenesis, DNA replication, andgene regulation.a) With the aid of a diagram, generally narrate how a foreign gene can be inserted into alambda insertion vector and subsequently infect an Escherichia coli cell.b) You are cloning a 7.5 kb gene into a lambda gt10 vector, utilizing a restriction site whichspecifically present in the vector. State the restriction site that you can use for this purposeand suggest a screening procedure to indicate successful integration of the gene into thehost genome.arrow_forwardThe NIH Recombinant DNA Advisory Committee has what responsibility? a) Approving all forms of human clinical gene therapy b) Identifying all major risks to the human subjects in a specific research protocol c )Reviewing clinical trials involving human gene transfer d) Analyzing genomes and determining whether they appear mutagenicarrow_forward(a) What is Figure 1 in the Zheng et al paper showing? Describe it in your own words. (b) What are some sections of the Zheng paper that are re-arranged or different than you might expect in a primary research article. Is this still primary research? (c) How did the author use CRISPR to alter the butterfly wing expression? What was the result of this use of CRISPR?arrow_forward
- A DNA library is aa) A DNA fragment inserted into a vector.b) A general collection of all genes sequenced thus far.c) All DNA fragments identified with a probe.d) A collection of DNA fragments that make up the entire genome of a particular organism.arrow_forwardWhat are the advantages of using organisms such as bacteria and yeast as cloning hosts? What safety considerations must be made when choosing a cloning host to make proteins for human use?arrow_forward1) Which of the following are referred to as retrotransposons? a) VNTRS b) SINES c) LINES d) STRS 2) Which of the following techniques relies on hybridation between complementary nucleic acid (DND or RNA)? a) Southern blot b) Northern blot c) d) None of the above are correct A+B 3) Cancer is often the result of activation of_ to and the inactivation of genes. a) "oncogenes, tumor-suppressor genes, proto-oncogenes" b) "proto-oncogenes, oncogenes, tumor-suppressor genes" c) "oncogenes, proto-oncogenes, tumor-suppressor genes" d) "proto-suppressor genes, suppressor, oncogenes"arrow_forward
- Choose the phrase that best describes the role of a cloning vector. A) Separates fragments of DNA B) Detects the presence of specific DNA sample C) Isolates DNA from a donor organism D) Screens for recombinants that have been successfully transformed E) Introduces DNA into a cloning hostarrow_forwardYou would like to amplify the gene shown below using PCR. What is the sequence of the primer you would generate for the left side of the sequence? a) 5’ GCGGTTAA 3’ b) 5’ CGCCAATT 3’ c) 5’ TTAACCGC 3’ d) 5’ AATTGGCG 3’arrow_forwardWhat would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License