Videos
Can aerobic bacteria grow in the absence of
To determine:
If aerobic bacteria can grow in the absence of oxygen andthe method to determine whether bacteria growing on a Petri plate from the brewer jar is anaerobes.
Introduction:
Bacteria is a single-celled microscopic organism that belongs to the prokaryotic domain. They show diversity in their niche and cellular features. Bacteria can survive in aerobic, anaerobic or both conditions (facultative anaerobes). Facultative anaerobes can switch mechanisms to adapt to the environment.
Explanation of Solution
Strict aerobes can only survive in the presence of oxygen, but they can thrive for a short duration in the absence of oxygen too. Some aerobes can switch mechanism to use another terminal electron acceptor instead of oxygen.
Brewer jar is an anaerobic chamber for culturing anaerobes. Atmospheric oxygen is removed from the jar by chemically combining it with hydrogen to release water. The bacteria growing in Petri plates from brewer jar can be tested for anaerobes by subculturing them in aerobic conditions or thioglycolate medium. The subculture of anaerobes would not be able to survive in aerobic conditions whereas thioglycolate broth is a nutrient-rich differential medium used for differentiating aerobic and anaerobic microorganisms. The presence of sodium thioglycolate in the medium allows the growth of obligate anaerobic organisms by consuming the dissolved oxygen. This results in the creation of an anaerobic environment at the bottom of the culture tube. Aerobic organisms thus accumulate at the top of the broth to obtain atmospheric oxygen whereas the obligate anaerobes accumulate at the bottom of the tube where the oxygen is completely absent.
The bacteria growing in Petri plates from brewer jar can be tested for anaerobes by subculturing them in aerobic conditions or thioglycolate medium.
Want to see more full solutions like this?
Chapter 19 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
- Why is a GasPak anaerobic jar necessary for the culture of anaerobes on Brewer’s anaerobic agar plates but not in tubes of fluid thioglycollate medium (FTM)?arrow_forwardPlease give me the general conclusion on the topic 'air pollution'. (1 to 2 pages)arrow_forwardThe nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks) a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w / v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .arrow_forward
- Please answer fastarrow_forwardExplain in paragraph form how putting salt on meat preserves it fromspoilage by bacteria. This is not a writing assignment. Please have the answer encoded and not handwritten.arrow_forwardName at least one reason that we might want to use a Winogradsky column rather than a laboratory medium such as nutrient agar or beef heart agar. Describe why metabolism in the presence of oxygen might cause problems for microorganisms.arrow_forward
- I need 21 terms pleasearrow_forwardIn paragraph form (about 2-3 long paragraphs), how would you characterize and identify antimicrobial agents from root samples using chromatography and electrophoresis? Please be in full details.arrow_forwardIn this section you will present the results of all the tests that was done to identify your organism Enterobacter aerogenes (your observation). In the laboratory we tabulated our results and created a chart (ID Matrix). Here you are describing the result of each test (in your own words). Include the name of the Indicator used (if any). Color changes that represent positive/negative results must be mentioned here. Your organism is a Gram-negative organism (Enterobacter aerogenes) You must discuss all the IMViC tests and Hydrogen sulfide test in DETAIL. 1. Indole test 2. Methyl red test 3. Vogues-Proskauer test 4. Citrate Test 5. Hydrogen Sulphide Testarrow_forward
- Why bacterium Thermus thermophilus is used ?arrow_forwardhttps://courses.lumenlearning.com/microbiology/chapter/controlling-microbial-growth/ Read this Article about Controlling Microbial Growth and answer the following questions: What are some characteristics of microbes and infectious agents that would require handling in a BSL-3 laboratory? What is the purpose of degerming? Does it completely eliminate microbes? What are some factors that alter the effectiveness of a disinfectant?arrow_forwardhttps://www.youtube.com/watch?time_continue=2&v=B_lHQSxz9GI&feature=emb_logo Using what you’ve learned from the video, answer the following questions in the spaces provided.1 Death is a major event not only for the person who dies but also for the microbes that inhabit their body. Which of the following statements best describes why? a. All the microbes that were living inside the person’s body die when the person dies. b. Microbial populations grow and spread to different areas of the body after the person dies. c. Microbes in the body have fewer resources after the person dies, which increases competition. 2 Which of the following statements about cadavers (dead bodies) is most likely to be true? a. Microbial communities in and around a cadaver change over time. b. Samples taken from different cadavers always contain the same microbes. c. The microbial community found in a cadaver is very similar to the one found in a living body. 3 After death, gases build up inside the…arrow_forward
