CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 19, Problem 1AL
Consider the fact that the use of 16S rRNA sequencing as a taxonomic and phylogenetic tool has resulted in tripling the number of bacterial phyla. Why has the advent of this genetic technique expanded the currently accepted number of microbial phyla?
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In studying Lokiarchaeota, researchers identified eukaryotic signature genes and used this information to better understand the relationship between archaeans and eukaryotes. Many other types of genetic analysis can be used that focus on certain types of
genes. One example of this is multilocus sequence typing (MLST), which focuses on examining genes called housekeeping genes. Microbes encode both housekeeping and pathogenic genes in their genome. Drag the examples of genes to the correct bins to
indicate whether they are housekeeping genes, pathogenic genes, or eukaryotic signature genes.
Place the phrase describing a gene product in the correct bin.
► View Available Hint(s)
peptidoglycan synthesis genes
Housekeeping genes
membrane remodeling
genes
cytoskeletal
genes
membrane lipid
genes
Pathogenic genes
endotoxin genes
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ribosomal genes exotoxin genes
Eukaryotic signature genes
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Let's say that you're a scientist trying to analyze newly sequenced bacterial DNA genomes from
an environmental sample of rainforest soil, and your first task is to organize all of the genomes
you find into a phylogenetic tree. If you couldn't use the 16S rRNA gene sequence to create the
tree, which of these alternative genes would work best for your task of creating a tree that works
for all the bacteria in your sample?
The gene for Bacteriochlorophyll A
A gene for an RNA polymerase component protein
The first gene in the histidine biosynthesis pathway
The first gene in the LPS synthesis pathway
I need the answer as soon as possible
Chapter 19 Solutions
CUSTOM PRESCOTT'S MICROBIOLOGY
Ch. 19.1 - What is a natural classification? What microbial...Ch. 19.1 - Prob. 2CCCh. 19.1 - Consider the finding that bacteria capable of...Ch. 19.2 - What is the difference between a microbial species...Ch. 19.2 - Why is it important to have a type strain for each...Ch. 19.2 - The genus Salmonella was once thought to contain...Ch. 19.3 - What are the advantages of using each major group...Ch. 19.3 - Why is rRNA so suitable for determining...Ch. 19.3 - Prob. 3CCCh. 19.4 - Could a phylotype be considered an OTU? What about...
Ch. 19.4 - List the differences between distance-based and...Ch. 19.4 - Prob. 3CCCh. 19.4 - You are building a tree based on 16S rRNA sequence...Ch. 19.4 - Is HGT involved in movement of genes in the core...Ch. 19.5 - On what evidence is this hypothesis based?Ch. 19.5 - Construct a scenario in which each of the...Ch. 19.5 - Define ecotype. Do you think it is necessary to...Ch. 19.5 - What is the difference between the core genome and...Ch. 19.5 - Of the following genes, which do you think are...Ch. 19.5 - Prob. 4CCCh. 19.6 - Prob. 1CCCh. 19.6 - Describe two different situations in which it...Ch. 19 - Prob. 1RCCh. 19 - Prob. 2RCCh. 19 - Prob. 3RCCh. 19 - Prob. 4RCCh. 19 - Prob. 5RCCh. 19 - Prob. 6RCCh. 19 - Consider the fact that the use of 16S rRNA...Ch. 19 - You have recently established a pure culture of a...Ch. 19 - Prob. 3ALCh. 19 - Prob. 4ALCh. 19 - Prob. 5ALCh. 19 - Prob. 6ALCh. 19 - Prob. 7AL
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- Give typing answer with explanation and conclusion In thinking about the clinical application of phylogenetics, discuss the role of phylogenetics in these areas. a) Predictions of drug resistance b) Identifying the origin of an outbreak c)Predicting the spread of a diseasearrow_forwardWhat are the 4 characteristics/properties of rRNA genes which make them good molecular chronometers. Why viruses are not found in the tree of life? Why might viable cell cultures be of more use in microbial taxonomy than preserved specimens? Is it possible to provide a formal name for a microorganism that has not been cultivated in isolation? What kind of name might be used if a microorganism is well-characterized but cannot yet be cultivated in isolation?arrow_forwardDescribe the Theory of Endosymbiosis. a) Briefly define the Endosymbiotic Theory. 2 pts,b) Explain why the Theory of Endosymbiosis is considered a “theory” and not a “hypothesis.” 3 pts,c) Provide a description of eukaryotic and prokaryotic cells, including at least 4 similarities and 4 structural differences. 4 pts,d) Describe the sequence of events with anaerobic ancestral proto-eukaryotes and aerobic prokaryotes that led to the formation of mitochondria and chloroplasts inside eukaryotic cells. (Be sure to define aerobic and anaerobic respiration.) 6 pts,e) Explain how each, the anaerobic ancestral proto-eukaryotes and aerobic prokaryotes, benefited from the relationship. 4 pts,f) Describe 7 pieces of evidence for this scientific theory. Some of this evidence will consist of a list of similarities between mitochondria characteristics and bacteria characteristics. Along with this list of similarities, explain HOW the characteristics are similar. 10 pts,g) The Endosymbiotic Theory…arrow_forward
- select the correct options: A) For organisms that diverged >74 mya, ignore 3rd base positions within codons because they will offer little phylogenetic information for organisms. B) use different parts ofthe genome to estimate different parts of a phylogeny C) Use only 1st base positions within codons because they increase rapidly at first, then level off as result of single subsitituions at the same sites D) Use slowly evolvong sites in the squecne, such as 2nd base positions within codonsfor analyzing the oldest relationships among organisms.arrow_forwardList three reasons why rRNA genes are suitable for phylogeneticanalyses.arrow_forwardDescribe the relationship between the original bacteria kingdom called monera and the domain called bacteria.arrow_forward
- What problem with the six-kingdom classification system was addressed by the three-domain classification system? How did it address the problem?arrow_forwardExplain why the answer is correct. For eukaryote cells to evolve into new types of eukaryotic organisms, which of the following would one expect to occur? The correct answer is E a) these cells have evolved because they have different DNA because of mutations b) Their endoplasmic reticulum is synthesizing new and/or different proteins. c) These specialized cells activate new genes to synthesize new proteins. d) these organisms have new characteristics because they have acquired new genes. e) all of these are truearrow_forwardThe fact that a prokaryote has the ability to make a new protein that is placed in the cell membrane of the mesosome is important to the process of evolution because this represents a) the mutation of a gene, b) new DNA producing a new genetic variation, c) r RNA using a new type of m RNA, d) the species having a greater degree of genetic variability, e) all are truearrow_forward
- You isolate bacteria from several different pools at the recreation center. Curious about which bacteria likely have a common ancestor with your reference pool (the Buffalo-pool), you BLAST the 16S ribosomal gene from the Buffalo pool bacteria against the 16S ribosomal genes from the other pools. The results are shown below. Which of the bacteria likely have a common ancestor with the Buffalo-pool bacteria? Use e as a cutoff for homology. Organism Dive-well bacteria Competition-pool bacterial Practice-pool bacteria Puddle bacteria Puddle bacteria Practice-pool bacteria Competition-pool bacteria Dive-well bacteria E-value 9e-175 -300 8e 4e-2 4arrow_forwardWhy did Carl Woese propose the domain Archaea? The domain Bacteria already had too many organisms in its classification. The methanogens were discovered to be a complex organism, between prokaryotic bacteria and eukaryotes, thus it needed its own domain. He discovered that methanogens were too large to be classified as bacteria, but too small to be classified as Eukarya. In analyzing the rRNA of methanogens, he discovered that it was completely distinct from any other bacterial RRNA that had been studied. The DNA of methanogens was discovered to use different nucleotides than those of organisms in Eukarya or Bacteria.arrow_forwardfind an example of a set of genes that have been horizontally gene transferred between bacteria or archaea. Which organisms are the genes transferred between? Explain the set of genes that were transferred. Include what they are for and what the advantage to sharing those genes are.What do the organisms use the genes for?Which organisms the transfer happened between?What might be the advantage be to share the genes with other organisms?arrow_forward
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