ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
4th Edition
ISBN: 9781265061173
Author: McKinley
Publisher: MCG CUSTOM
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Chapter 19, Problem 17DYKB
Describe the functional differences in the effects of the sympathetic and parasympathetic divisions of the autonomic nervous system on the activity of the heart.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 19 Solutions
ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
Ch. 19.1 - Prob. 1WDYLCh. 19.1 - Prob. 2WDYLCh. 19.1 - Prob. 3WDYLCh. 19.1 - Prob. 4WDYLCh. 19.1 - Prob. 5WDYLCh. 19.2 - What is the bony structure that protects both the...Ch. 19.2 - Prob. 7WDYLCh. 19.2 - Prob. 8WDYLCh. 19.3 - Prob. 9WDYLCh. 19.3 - Prob. 10WDYL
Ch. 19.3 - Prob. 11WDYLCh. 19.3 - Prob. 12WDYLCh. 19.3 - Prob. 13WDYLCh. 19.3 - Prob. 14WDYLCh. 19.3 - Prob. 15WDYLCh. 19.3 - Prob. 16WDYLCh. 19.3 - What areas of the heart are deprived of blood when...Ch. 19.3 - Prob. 18WDYLCh. 19.4 - Prob. 19WDYLCh. 19.5 - Prob. 20WDYLCh. 19.5 - Which autonomic division is associated with the...Ch. 19.6 - Prob. 22WDYLCh. 19.6 - What is autorhythmicity? Describe how nodal cells...Ch. 19.6 - What is the path of an action potential through...Ch. 19.6 - What anatomic features slow the conduction rate of...Ch. 19.7 - In which direction does Ca2+ move in response to...Ch. 19.7 - What three electrical events occur at the...Ch. 19.7 - What is the significance of the extended...Ch. 19.7 - What events in the heart are indicated by each of...Ch. 19.8 - Pressure changes that occur during the cardiac...Ch. 19.8 - What is occurring during ventricular ejection?Ch. 19.8 - Prob. 32WDYLCh. 19.8 - Define end-diastolic volume, end-systolic volume,...Ch. 19.9 - What are the two factors that determine cardiac...Ch. 19.9 - What is the cardiac output at rest and during...Ch. 19.9 - Prob. 36WDYLCh. 19.9 - Describe the atrial reflex, which involves...Ch. 19.9 - Prob. 38WDYLCh. 19.9 - Prob. 39WDYLCh. 19.10 - What would be the path of blood flow through the...Ch. 19 - Which of the following is the correct circulatory...Ch. 19 - The pericardial cavity is located between the a....Ch. 19 - How is blood prevented from backflowing from the...Ch. 19 - ____ 4. Venous blood draining from the heart wall...Ch. 19 - _____ 5. Calcium channels in the nodal cells...Ch. 19 - ____6. Action potentials are spread rapidly...Ch. 19 - Why is it necessary to stimulate papillary muscles...Ch. 19 - ____ 8. Preload is a measure of a. stretch of...Ch. 19 - ____ 9. All of the following occur when the...Ch. 19 - ____10. What occurs during the atrial reflex? a....Ch. 19 - Prob. 11DYKBCh. 19 - Compare the structure, location, and function of...Ch. 19 - Prob. 13DYKBCh. 19 - Explain why the walls of the atria are thinner...Ch. 19 - Describe the structure and function of...Ch. 19 - Explain the general location and function of...Ch. 19 - Describe the functional differences in the effects...Ch. 19 - Prob. 18DYKBCh. 19 - List the five events of the cardiac cycle, and...Ch. 19 - Define cardiac output, and explain how it is...Ch. 19 - A young man was doing some vigorous exercise when...Ch. 19 - A young man was doing some vigorous exercise when...Ch. 19 - Prob. 3CALCh. 19 - Prob. 4CALCh. 19 - During surgery, the right vagus nerve was...Ch. 19 - Prob. 1CSLCh. 19 - Prob. 2CSLCh. 19 - Your grandfather was told that his SA node...
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