The consequences of radio labeling with 1 4 CO 2 in the reductive TCA cycle should be determined. Concept Introduction: In the forward cycle, CO 2 will be lost whereas in reverse TCA cycle it will be incorporated which will happen at the conversion of succinyl−CoA to α -ketoglutarate and again at the conversion of isocitrate to citrate.

Question

9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2

Answer 1

Question

Chapter 19, Problem 12P

Interpretation Introduction

Interpretation:

The consequences of radio labeling with ¹⁴CO₂ in the reductive TCA cycle should be determined.

Concept Introduction:

In the forward cycle, CO₂ will be lost whereas in reverse TCA cycle it will be incorporated which will happen at the conversion of succinyl−CoA to α -ketoglutarate and again at the conversion of isocitrate to citrate.

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