Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Textbook Question
Chapter 18.4, Problem 3QC
Describe the properties of the three types of capillaries, and explain how they differ.
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1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Chapter 18 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 18.1 - Define the three types of blood vessels in the...Ch. 18.1 - Prob. 2QCCh. 18.1 - Prob. 3QCCh. 18.1 - Prob. 4QCCh. 18.1 - Prob. 5QCCh. 18.1 - 6. How do veins differ structurally and...Ch. 18.1 - Prob. 7QCCh. 18.1 - What are venous valves, and what are their...Ch. 18.1 - Prob. 9QCCh. 18.2 - Prob. 1QC
Ch. 18.2 - Prob. 2QCCh. 18.2 - Prob. 3QCCh. 18.2 - Prob. 4QCCh. 18.2 - Prob. 5QCCh. 18.2 - Prob. 6QCCh. 18.2 - 7. How does mean arterial pressure differ from...Ch. 18.2 - Prob. 8QCCh. 18.2 - Prob. 9QCCh. 18.3 - Prob. 1QCCh. 18.3 - Prob. 2QCCh. 18.3 - Prob. 3QCCh. 18.3 - Prob. 4QCCh. 18.3 - Prob. 5QCCh. 18.3 - Prob. 6QCCh. 18.3 - Prob. 7QCCh. 18.3 - 8. What is circulatory shock, and why is it...Ch. 18.4 - Describe the structure and size of a typical...Ch. 18.4 - 2. List three ways in which substances may cross...Ch. 18.4 - 3. Describe the properties of the three types of...Ch. 18.4 - 4. What is tissue perfusion?
Ch. 18.4 - Prob. 5QCCh. 18.4 - Prob. 6QCCh. 18.4 - Prob. 7QCCh. 18.4 - Prob. 8QCCh. 18.4 - Prob. 9QCCh. 18.5 - What is hydrostatic pressure? How does hydrostatic...Ch. 18.5 - 2. In which direction does the hydrostatic...Ch. 18.5 - 3. What is colloid osmotic pressure? In which...Ch. 18.5 - Prob. 4QCCh. 18.5 - Where in the capillary does net filtration take...Ch. 18.5 - Prob. 6QCCh. 18.6 - List the three branches of the aortic arch.Ch. 18.6 - Prob. 2QCCh. 18.6 - 3. What is the cerebral arterial circle, and what...Ch. 18.6 - Prob. 4QCCh. 18.6 - Which arteries supply the anterior and posterior...Ch. 18.6 - Prob. 6QCCh. 18.6 - Which artery supplies the upper limb?Ch. 18.6 - Trace the arterial supply of the upper limb from...Ch. 18.6 - 9. Which artery supplies the lower limb?
Ch. 18.6 - Trace the arterial supply of the lower limb from...Ch. 18.6 - Prob. 11QCCh. 18.7 - Where do most veins superior to the diaphragm...Ch. 18.7 - Prob. 2QCCh. 18.7 - Where are the dural sinuses located? What drains...Ch. 18.7 - How does drainage of the posterior body wall...Ch. 18.7 - 5. Which abdominal vessels drain straight into...Ch. 18.7 - Prob. 6QCCh. 18.7 - Prob. 7QCCh. 18.7 - Prob. 8QCCh. 18.7 - Prob. 9QCCh. 18.7 - Prob. 10QCCh. 18 - Prob. 1CYRCh. 18 - Locations where vessels connect via collateral...Ch. 18 - 3. The carotid sinus contains:
a....Ch. 18 - 4. Which of the following factors would increase...Ch. 18 - Which of the following would produce a decrease in...Ch. 18 - Fill in the blanks: The two pressures within the...Ch. 18 - The lowest pressure in the systemic circuit occurs...Ch. 18 - Explain the mechanisms that assist in the return...Ch. 18 - Mark the following statements as true or false. If...Ch. 18 - Prob. 10CYRCh. 18 - Capillaries consist of: a. three thin tunics. b....Ch. 18 - List three ways in which substances can cross the...Ch. 18 - Which of the following structures is the leakiest?...Ch. 18 - Prob. 14CYRCh. 18 - 15. The hydrostatic pressure gradient drives water...Ch. 18 - Prob. 16CYRCh. 18 - Match the following arteries with the correct...Ch. 18 - Which of the following is not a common pulse...Ch. 18 - 19. Which of the following vessels does not drain...Ch. 18 - Match the following veins with the correct...Ch. 18 - 1. Explain why a severed artery spurts blood,...Ch. 18 - 2. Explain why a person who is 7 feet tall is...Ch. 18 - Prob. 3CYUCh. 18 - Prob. 1AYKACh. 18 - 2. Mr. Gupta has been diagnosed with nephrotic...Ch. 18 - Predict the effects of each of the following on...Ch. 18 - Prob. 4AYKBCh. 18 - Ms. Rodgers has been diagnosed with secretion of...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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