PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Textbook Question
Chapter 18.2, Problem 3CC
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Why does reversible chain termination sequencing yield short reads?
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Chapter 18 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4CCCh. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...Ch. 18.3 - Retrieve, Infer, Apply Examine figure 18.8. How...
Ch. 18.4 - Prob. 1MICh. 18.4 - Prob. 1CCCh. 18.4 - Prob. 2CCCh. 18.4 - Prob. 3CCCh. 18.5 - Figure 18.12 Metabolic Pathways and Transport...Ch. 18.5 - Prob. 2MICh. 18.5 - Prob. 3MICh. 18.5 - Prob. 1CCCh. 18.5 - Retrieve, Infer, Apply How might the following...Ch. 18.5 - Retrieve, Infer, Apply Compare and contrast...Ch. 18.5 - Retrieve, Infer, Apply Why does two-dimensional...Ch. 18.5 - Retrieve, Infer, Apply What is the difference...Ch. 18.5 - Retrieve, Infer, Apply Describe a ChIP-Seq...Ch. 18.7 - Prob. 1MICh. 18.7 - Retrieve, Infer, Apply Cite an infectious disease...Ch. 18.7 - Prob. 2CCCh. 18.7 - Prob. 3CCCh. 18 - Prob. 1RCCh. 18 - Prob. 2RCCh. 18 - Prob. 3RCCh. 18 - Prob. 4RCCh. 18 - Prob. 5RCCh. 18 - Prob. 1ALCh. 18 - Prob. 2ALCh. 18 - You are developing a new vaccine for a pathogen....Ch. 18 - Prob. 4AL
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- what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the genome sequence.arrow_forwardDescribe two different types of protein microarrays, and discuss their uses.arrow_forwardCompare 2D polyacrylamide gel electrophoresis to LC-MS as a proteomics technique to identify differences between patients with a disease and controls without the disease.arrow_forward
- Experiment A microarray was hybridized with a mixture of two differentially labeled fluorescent cDNAs, one prepared using retinal RNA of 1-day-old mice (labeled with a green fluorescent dye) and the other prepared using retinal RNA of 28-day-old mice (red fluorescence). The two probes were prepared from identical amounts of retinal tissues and were mixed together for hybridization to the microarray. Unhybridized probes were washed away, and the microarray was photographed in a fluorescence microscope.arrow_forwardList the critical steps in the ribosome profiling protocol and explain what this method is useful for when coupled with high-throughput sequencing.arrow_forwardRestriction endonuclease digestion of a DNA sequence yielded fragments of the following sizes: 1. 5.2 kb 2. 0.8 kb 3. 1.2 kb 4. 3.8 kb 5. 3.1 kb After gel electrophoresis, what would be the order in which these fragments would be found—the last fragment listed being furthest from the negative pole.arrow_forward
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- Givearrow_forwardNeed bioinformatics help. 1) Examine the quality of the raw sequencing data 2) Trim low quality sequences and sequencing adapters from the raw reads 3) Align trimmed reads against the reference genome 4) Count number of reads mapped per annotated gene 5) Differential expression analysis 6) Visualize sequencing reads aligned to the reference genome 7) Gene set enrichment analysis (GSEA) Choices: Google Colab UCSC Genome Browser fasterq-dump MultiQC BLAST DESeq2 STAR Gorilla featureCounts SNPEff samtools flagstat BWA mem Integrative Genome Viewer (IGV) LoFreq Fastp FastQC align.seq 2) Trim low quality sequences and sequencing adapters from the raw reads 3) Align trimmed reads against the reference genome 4) Count number of reads mapped per annotated gene 5) Differential expression analysis 6) Visualize sequencing reads aligned to the reference genome 7) Gene set enrichment analysis (GSEA) Choices: Google Colab UCSC Genome Browser…arrow_forwardAside from gel electrophoresis Give another method to quantify DNA. Explain the concept behind this method.arrow_forward
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