R1 and Su B mutations are intragenic suppressors or intergenic suppressors. Introduction: Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su B . R1 is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

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Answer 1

Question

Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and Su^B mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su^B. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su^B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

b.

Summary Introduction

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.

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19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Match the letter labels in the figure below to the terms. Some letter labels are not used. MNNNNNNIN M C B A M D F E H K G 8

The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions. 1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction? 2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe: a) What molecule do you recommend for the trial. Why? b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for? 3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale. 4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale. Mutation 1 Mutation 2

Answer 2

Question

Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and Su^B mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su^B. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su^B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

b.

Summary Introduction

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.

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Answer 3

Question

Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and Su^B mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su^B. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su^B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

b.

Summary Introduction

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.

Expert Solution & Answer

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Answer 4

Question

Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and Su^B mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su^B. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su^B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

b.

Summary Introduction

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.

Expert Solution & Answer

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Answer 5

Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and Su^B mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su^B. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su^B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

b.

Summary Introduction

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.

Answer 6

Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and Su^B mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su^B. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su^B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

Answer 7

Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and Su^B mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and Su^B. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation Su^B is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

Answer 8

b.

Summary Introduction

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.

Answer 9

b.

Summary Introduction

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 18.1 - Prob. 1CC Ch. 18.1 - Prob. 2CC Ch. 18.1 - Prob. 3CC Ch. 18.1 - Prob. 1COQ Ch. 18.1 - Prob. 2COQ Ch. 18.1 - Prob. 3COQ Ch. 18.1 - Prob. 4COQ Ch. 18.1 - Prob. 17AQP Ch. 18.1 - Prob. 18AQP Ch. 18.1 - Prob. 19AQP

To explain: The way in which R1 and SuB restore the ability of araB14 to metabolize arabinose and why SuB is able to more fully restore this ability.

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Chapter 18 Solutions

To explain:

The way in which R1 and Su^B restore the ability of araB14 to metabolize arabinose and why Su^B is able to more fully restore this ability.