Biological Science (7th Edition)
Biological Science (7th Edition)
7th Edition
ISBN: 9780134678320
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Greg Podgorski, Emily Taylor, Jeff Carmichael
Publisher: PEARSON
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Chapter 18, Problem 8TYU
Summary Introduction

To review:

The effect of the addition of the Isopropyl β-D-1-thiogalactopyranoside (IPTG) to the Escherichia coli growth medium containing no glucose or lactose on lac operon regulation.

Introduction:

The IPTG is a structure quite similar to the lactose molecule. It can be transported by the galactoside permease and is capable of binding to the lac repressor protein. Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a molecule with structure resemblance to lactose. IPTG can be transported into cells by galactoside permease and can bind to the lac repressor protein. However, unlike lactose, IPTG is not broken down by β-galactosidase.

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