Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 18, Problem 7BYMV
State a meaning of each word element and give a medical term from this chapter that uses it or a slight variation of it.
7. natri-
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 18 Solutions
Human Anatomy
Ch. 18.1 - Define the word hormone. Compare hormones and...Ch. 18.1 - Prob. 2BYGOCh. 18.1 - Prob. 3BYGOCh. 18.1 - How do the nervous and endocrine systems differ?...Ch. 18.2 - Prob. 5BYGOCh. 18.2 - Prob. 6BYGOCh. 18.2 - Prob. 7BYGOCh. 18.2 - Prob. 8BYGOCh. 18.3 - The zona fasciculate thickens significantly in...Ch. 18.3 - Often, two hormones have opposite (antagonistic)...
Ch. 18.3 - Prob. 9BYGOCh. 18.3 - Prob. 10BYGOCh. 18.3 - Prob. 11BYGOCh. 18.3 - What is the difference between a gonadal hormone...Ch. 18.3 - What hormones are most important in regulating...Ch. 18.3 - Prob. 14BYGOCh. 18.4 - Prob. 15BYGOCh. 18.4 - Prob. 16BYGOCh. 18.4 - Prob. 17BYGOCh. 18.4 - Prob. 18BYGOCh. 18 - The definitions of hormone and endocrine systemCh. 18 - Prob. 18.1.2AYLOCh. 18 - Prob. 18.1.3AYLOCh. 18 - Prob. 18.1.4AYLOCh. 18 - Prob. 18.1.5AYLOCh. 18 - Prob. 18.1.6AYLOCh. 18 - Prob. 18.1.7AYLOCh. 18 - Prob. 18.2.1AYLOCh. 18 - Prob. 18.2.2AYLOCh. 18 - Prob. 18.2.3AYLOCh. 18 - Prob. 18.2.4AYLOCh. 18 - Prob. 18.2.5AYLOCh. 18 - Prob. 18.2.6AYLOCh. 18 - Prob. 18.2.7AYLOCh. 18 - The target organs or tissues and the funtions of...Ch. 18 - Prob. 18.2.9AYLOCh. 18 - Prob. 18.3.1AYLOCh. 18 - Prob. 18.3.2AYLOCh. 18 - The location, anatomy, and histology of the...Ch. 18 - Prob. 18.3.4AYLOCh. 18 - Prob. 18.3.5AYLOCh. 18 - Prob. 18.3.6AYLOCh. 18 - Prob. 18.3.7AYLOCh. 18 - The location and structure of the pancreatic...Ch. 18 - Prob. 18.3.9AYLOCh. 18 - Prob. 18.3.10AYLOCh. 18 - Prob. 18.4.1AYLOCh. 18 - Prob. 18.4.2AYLOCh. 18 - Prob. 18.4.3AYLOCh. 18 - The cause and effects of pheochromocytoma of the...Ch. 18 - The cause and effects of endemic goiterCh. 18 - Prob. 18.4.6AYLOCh. 18 - Prob. 1TYRCh. 18 - Prob. 2TYRCh. 18 - Prob. 3TYRCh. 18 - Prob. 4TYRCh. 18 - Prob. 5TYRCh. 18 - Prob. 6TYRCh. 18 - Prob. 7TYRCh. 18 - Prob. 8TYRCh. 18 - Which of these endocrine glands is most directly...Ch. 18 - Prob. 10TYRCh. 18 - Prob. 11TYRCh. 18 - Prob. 12TYRCh. 18 - Prob. 13TYRCh. 18 - Prob. 14TYRCh. 18 - Prob. 15TYRCh. 18 - Prob. 16TYRCh. 18 - Prob. 17TYRCh. 18 - Prob. 18TYRCh. 18 - Prob. 19TYRCh. 18 - Prob. 20TYRCh. 18 - Prob. 1BYMVCh. 18 - Prob. 2BYMVCh. 18 - Prob. 3BYMVCh. 18 - Prob. 4BYMVCh. 18 - Prob. 5BYMVCh. 18 - Prob. 6BYMVCh. 18 - State a meaning of each word element and give a...Ch. 18 - Prob. 8BYMVCh. 18 - Prob. 9BYMVCh. 18 - Prob. 10BYMVCh. 18 - Prob. 1TOFCh. 18 - Prob. 2TOFCh. 18 - Prob. 3TOFCh. 18 - Prob. 4TOFCh. 18 - Prob. 5TOFCh. 18 - Prob. 6TOFCh. 18 - Prob. 7TOFCh. 18 - Prob. 8TOFCh. 18 - Prob. 9TOFCh. 18 - Prob. 10TOFCh. 18 - Prob. 1TYCCh. 18 - Examine the anatomical relationship between the...Ch. 18 - Prob. 3TYCCh. 18 - Prob. 4TYCCh. 18 - Prob. 5TYC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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- Why are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forward
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