Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 18, Problem 18.3.8AYLO
The location and structure of the pancreatic islets; the three primary types of islet cells; the hormone secreted by each cell type; and the functions of those hormones
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 18 Solutions
Human Anatomy
Ch. 18.1 - Define the word hormone. Compare hormones and...Ch. 18.1 - Prob. 2BYGOCh. 18.1 - Prob. 3BYGOCh. 18.1 - How do the nervous and endocrine systems differ?...Ch. 18.2 - Prob. 5BYGOCh. 18.2 - Prob. 6BYGOCh. 18.2 - Prob. 7BYGOCh. 18.2 - Prob. 8BYGOCh. 18.3 - The zona fasciculate thickens significantly in...Ch. 18.3 - Often, two hormones have opposite (antagonistic)...
Ch. 18.3 - Prob. 9BYGOCh. 18.3 - Prob. 10BYGOCh. 18.3 - Prob. 11BYGOCh. 18.3 - What is the difference between a gonadal hormone...Ch. 18.3 - What hormones are most important in regulating...Ch. 18.3 - Prob. 14BYGOCh. 18.4 - Prob. 15BYGOCh. 18.4 - Prob. 16BYGOCh. 18.4 - Prob. 17BYGOCh. 18.4 - Prob. 18BYGOCh. 18 - The definitions of hormone and endocrine systemCh. 18 - Prob. 18.1.2AYLOCh. 18 - Prob. 18.1.3AYLOCh. 18 - Prob. 18.1.4AYLOCh. 18 - Prob. 18.1.5AYLOCh. 18 - Prob. 18.1.6AYLOCh. 18 - Prob. 18.1.7AYLOCh. 18 - Prob. 18.2.1AYLOCh. 18 - Prob. 18.2.2AYLOCh. 18 - Prob. 18.2.3AYLOCh. 18 - Prob. 18.2.4AYLOCh. 18 - Prob. 18.2.5AYLOCh. 18 - Prob. 18.2.6AYLOCh. 18 - Prob. 18.2.7AYLOCh. 18 - The target organs or tissues and the funtions of...Ch. 18 - Prob. 18.2.9AYLOCh. 18 - Prob. 18.3.1AYLOCh. 18 - Prob. 18.3.2AYLOCh. 18 - The location, anatomy, and histology of the...Ch. 18 - Prob. 18.3.4AYLOCh. 18 - Prob. 18.3.5AYLOCh. 18 - Prob. 18.3.6AYLOCh. 18 - Prob. 18.3.7AYLOCh. 18 - The location and structure of the pancreatic...Ch. 18 - Prob. 18.3.9AYLOCh. 18 - Prob. 18.3.10AYLOCh. 18 - Prob. 18.4.1AYLOCh. 18 - Prob. 18.4.2AYLOCh. 18 - Prob. 18.4.3AYLOCh. 18 - The cause and effects of pheochromocytoma of the...Ch. 18 - The cause and effects of endemic goiterCh. 18 - Prob. 18.4.6AYLOCh. 18 - Prob. 1TYRCh. 18 - Prob. 2TYRCh. 18 - Prob. 3TYRCh. 18 - Prob. 4TYRCh. 18 - Prob. 5TYRCh. 18 - Prob. 6TYRCh. 18 - Prob. 7TYRCh. 18 - Prob. 8TYRCh. 18 - Which of these endocrine glands is most directly...Ch. 18 - Prob. 10TYRCh. 18 - Prob. 11TYRCh. 18 - Prob. 12TYRCh. 18 - Prob. 13TYRCh. 18 - Prob. 14TYRCh. 18 - Prob. 15TYRCh. 18 - Prob. 16TYRCh. 18 - Prob. 17TYRCh. 18 - Prob. 18TYRCh. 18 - Prob. 19TYRCh. 18 - Prob. 20TYRCh. 18 - Prob. 1BYMVCh. 18 - Prob. 2BYMVCh. 18 - Prob. 3BYMVCh. 18 - Prob. 4BYMVCh. 18 - Prob. 5BYMVCh. 18 - Prob. 6BYMVCh. 18 - State a meaning of each word element and give a...Ch. 18 - Prob. 8BYMVCh. 18 - Prob. 9BYMVCh. 18 - Prob. 10BYMVCh. 18 - Prob. 1TOFCh. 18 - Prob. 2TOFCh. 18 - Prob. 3TOFCh. 18 - Prob. 4TOFCh. 18 - Prob. 5TOFCh. 18 - Prob. 6TOFCh. 18 - Prob. 7TOFCh. 18 - Prob. 8TOFCh. 18 - Prob. 9TOFCh. 18 - Prob. 10TOFCh. 18 - Prob. 1TYCCh. 18 - Examine the anatomical relationship between the...Ch. 18 - Prob. 3TYCCh. 18 - Prob. 4TYCCh. 18 - Prob. 5TYC
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