MICROBIOLOGY-MASTERING MICRO.-ACCESS
11th Edition
ISBN: 9780321802705
Author: Tortora
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 18, Problem 6R
Summary Introduction
To determine:
ELISA is an immunological technique to detect antigen and antibody for particular disease.
Concept introduction: Direct and Indirect method are used to identify the antigen and antibody respectively.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Explain the limitations of the ELISA for HIV testing. What might cause a false positive result? What might cause a false negative result?
To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay).
1.In the "ELISA sandwich," what is the "meat"?
2.Both patients A and B had positive results. Why are these two patients' outcomes so different?
3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?
Briefly describe the principles and give an example of the use of aspecific test using immunoelectrophoresis, Western blot, complementfixation, fluorescent testing (direct and indirect), and immunoassays(direct and indirect ELISA).
Chapter 18 Solutions
MICROBIOLOGY-MASTERING MICRO.-ACCESS
Ch. 18 - Which could cause the disease it is supposed to...Ch. 18 - Define the following terms, and give an example of...Ch. 18 - DRAW IT Label the components of the direct and...Ch. 18 - How are monoclonal antibodies produced? What is...Ch. 18 - Explain the effects of excess antigen and antibody...Ch. 18 - Prob. 6RCh. 18 - How does the antigen in an agglutination reaction...Ch. 18 - Match the following serological tests in column A...Ch. 18 - Prob. 9RCh. 18 - Prob. 10R
Ch. 18 - What problems are associated with the use of live...Ch. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 1MCQCh. 18 - Prob. 2MCQCh. 18 - Prob. 3MCQCh. 18 - Prob. 4MCQCh. 18 - Prob. 5MCQCh. 18 - Prob. 6MCQCh. 18 - Prob. 7MCQCh. 18 - Test used to identify rabies virus in the brain of...Ch. 18 - Prob. 9MCQCh. 18 - Prob. 10MCQCh. 18 - Which of the following is proof of a disease...Ch. 18 - Prob. 2CAECh. 18 - Prob. 3CAECh. 18 - Prob. 4CAE
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a. Seropositivity means having a blood serum that tests positive. Explain why it may or may not develop at the same rate in all patients exposed to the same microbe. b. Would a high rate of false-positives decrease the sensitivity or specificity of a serological test?arrow_forwardName two types of immunodiffusion tests and describe how they operatearrow_forwardDescribe the principles behind direct and indirect fl uorescent antibody tests.arrow_forward
- How is HLA serologic testing performed? Question 28 options: a) HLA antigens move through a limited pH gradient and are immobilized, complexed with antibody, and stained. b) T lymphocytes are harvested and introduced to a panel of test sera specific for antigens of the HLA system. c) DNA is isolated from peripheral white blood cells, fragmented, and hybridized. d) B lymphocytes are harvested and introduced to a panel of test sera specific for antigens of the HLA system.arrow_forwardCompare and contrast Radial Immunodiffusion with it’s close relative, the Ouchterlony plate technique. How are they similar and how are they different?arrow_forwardDetermine the cell of the immune system responsible for processing antigen presented in a patch test.arrow_forward
- state the significance of heterophile antibodies in serological testingarrow_forwardHow is RT PCR used to detect Ebola? How does the actual RT PCR procedure work? How is ELISA used to detect Ebola? What is the method? What binds to what? How is Immunohistochemistry (IHC) used to detect Ebola? What is the method? What tissue is used, what binds to what?arrow_forwardWhen a patient exhibits a four-fold or greater rise in antibody titer against a specific microbial antigen between acute-phase and convalescent-phase serum samples, this is considered to be a “seroconversion”. Which of the following would be a correct interpretation of a seroconversion? what option below is the answer? None of the other four answers are a correct interpretation of a seroconversion These results show that the microbe itself has been detected in the patient’s sample(s) These findings cannot be interpreted because antibodies aren’t specific enough for antigens There is no evidence of current active infection because that would require that the antibody titer is decreasing rather than increasing The results show evidence of current or recent infection by the specific microbe because there has been an active immunologic response to its antigenarrow_forward
- Briefly describe how viral antigens can be screened for using an enzyme-linked immunosorbent assay (ELISA).arrow_forwardYou are presented with the following clinical scenario: "A 12 month old male child presents with recurrent bacterial infections. Flow cytometry shows a complete lack of B- lymphocytes." Using your knowledge of immunology and flow cytometry, identify which dot plots represent the healthy control and patient samples in each scenario and come up with a clinical diagnosis. Note that you may need to use additional background sources to fully understand what is going on at the cellular level for each diagnosis. What is the clinical diagnosis O HIV/AIDS O COVID-19 O LAD type 1 U E CD4-FITC CD8-PE CD45-PE CD8-PE 70% 13% 52% 10% 82% 5% 27% 5% CD8-PE CD4-FITC CD20-FITC CD4-FITC 17% 38% 13% 68% Agammaglobulinaemia CD45-PE CD4-FITC CD45-PE CD45-PE 35% 3% 62% 8% 93% 22% CD15-FITC CD8-PE CD20-FITC CD15-FITC Select the flow cytometry dot plot that corresponds to the healthy control sample Select the flow cytometry dot plot that corresponds to the patient sample 62% 30% 0% 77%arrow_forwardWhat is the effect of not adding the primary antibody to the ELISA reaction?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
