Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 18, Problem 5MCQ
Summary Introduction
Introduction:
Brucella is a gram negative bacterium that causes Brucellosis; it is present in milk. ELISA is technique which is used to detect the Brucella antigen, and IgG and IgM antibodies present in the Brucellosis patient serum sample. Direct ELISA test refers the identification of the antigen. But, Indirect ELISA test refers to the identification of the antibody present in the serum sample of AIDS patients. Here, Brucella is the antigen.
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Your goal is to produce black seeds resistant to mold. So you make the same cross again (between a homozygous black seeded, mold susceptible parent and a homozygous white seeded and mold resistant parent), and, again, advance progeny by SSD to create 100 F10 generation plants.
Based on the information you obtained from your first crossing experiment (Question #4), how many F10 plants would you expect to have black seeds and be resistant to mold?
Assume that a toxin produced by the mold fungus has been isolated. Only mold resistant seeds will germinate in the presence of the toxin. Could you use this toxin screening procedure to have segregation distortion work in your favor in the F2 generation? Explain your answer.
Info from Question 4
a. P Locus (Seed Color):
Hypothesis: The null hypothesis (H₀) is that seed color is controlled by alleles at a single locus.
Observed Data:
Total white seeds: 45 (resistant plants) + 6 (susceptible plants) = 51
Total black seeds: 7 (resistant…
10. Consider the following enzyme and its substrate where the "+" and "-" indicate cations and
anions, respectively. Explain which of the following inhibitors could inhibit this enzyme?
Which type of inhibitor would it be and why? (Video 5-2)
Substrate
Enzyme
Potential inhibitors
Using Punnett Squares Punnett squares are one good
way to predict the outcome of genetic crosses. Punnett
squares use mathematical probability to help predict the
genotype and phenotype combinations in genetic crosses. The
number of possible alleles from each parent determines the
number of rows and columns in the Punnett square.
Independent Assortment
KEY QUESTION How do alleles segregate when more than
one gene is involved?
Mendel wondered if the segregation of one pair of alleles
affects another pair. For example, does the gene that
determines the shape of a seed affect the gene for seed color?
This type of experiment is known as a two-factor, or dihybrid,
cross because it involves two different genes. Single-gene
crosses are monohybrid crosses.
Visual Reading Tool: Two-Factor Cross: F₂
The Punnett square shows the
results of self-crossing the F,
generation of a cross between
round yellow peas and wrinkled
green peas.
1. List the different genotypes in
the F, generation. What is the…
Chapter 18 Solutions
Microbiology: An Introduction
Ch. 18 - Which could cause the disease it is supposed to...Ch. 18 - Define the following terms, and give an example of...Ch. 18 - DRAW IT Label the components of the direct and...Ch. 18 - How are monoclonal antibodies produced? What is...Ch. 18 - Explain the effects of excess antigen and antibody...Ch. 18 - Prob. 6RCh. 18 - How does the antigen in an agglutination reaction...Ch. 18 - Match the following serological tests in column A...Ch. 18 - Prob. 9RCh. 18 - Prob. 10R
Ch. 18 - What problems are associated with the use of live...Ch. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 1MCQCh. 18 - Prob. 2MCQCh. 18 - Prob. 3MCQCh. 18 - Prob. 4MCQCh. 18 - Prob. 5MCQCh. 18 - Prob. 6MCQCh. 18 - Prob. 7MCQCh. 18 - Test used to identify rabies virus in the brain of...Ch. 18 - Prob. 9MCQCh. 18 - Prob. 10MCQCh. 18 - Which of the following is proof of a disease...Ch. 18 - Prob. 2CAECh. 18 - Prob. 3CAECh. 18 - Prob. 4CAE
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