Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
13th Edition
ISBN: 9780135789377
Author: Gerard Tortora, Berdell Funke
Publisher: PEARSON+
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Question
Chapter 18, Problem 4MCQ
Summary Introduction
Introduction:
ELISA (enzyme-linked immunosorbent assay) Test is used to determine and measure the antibodies in the blood. It is of two types, direct ELISA test, and indirect ELISA test. This test determines the infection for which antibodies are present in the blood.
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An ELISA was performed on the sera of three patients to detect antibody against varicella
(the chickenpox). Give the titer results shown in the answers, which patient's serum had the
most anti-varicella antibody?
There is not enough information to answer the question.
Patient 2, dilution 1:30
O Patient 1, dilution 1:2,000
O Patient 2, dilution 1:400
Figure 1. Cytotoxicity test on S96 well plates that was monitored by ELISA reader
3- Discussion
1- Explain another methods by which cytotoxicity is measured ?
Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?
Chapter 18 Solutions
Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
Ch. 18 - Which could cause the disease it is supposed to...Ch. 18 - Define the following terms, and give an example of...Ch. 18 - DRAW IT Label the components of the direct and...Ch. 18 - How are monoclonal antibodies produced? What is...Ch. 18 - Explain the effects of excess antigen and antibody...Ch. 18 - Prob. 6RCh. 18 - How does the antigen in an agglutination reaction...Ch. 18 - Match the following serological tests in column A...Ch. 18 - Prob. 9RCh. 18 - Prob. 10R
Ch. 18 - Prob. 1MCQCh. 18 - Prob. 2MCQCh. 18 - Prob. 3MCQCh. 18 - Prob. 4MCQCh. 18 - Prob. 5MCQCh. 18 - Prob. 6MCQCh. 18 - Prob. 7MCQCh. 18 - Test used to identify rabies virus in the brain of...Ch. 18 - Prob. 9MCQCh. 18 - Prob. 10MCQCh. 18 - What problems are associated with the use of live...Ch. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Which of the following is proof of a disease...Ch. 18 - Prob. 2CAECh. 18 - Prob. 3CAE
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