BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
3rd Edition
ISBN: 9781260670929
Author: Hoefnagels
Publisher: MCG CUSTOM
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Chapter 17.6, Problem 1MC
What features do all annelids share?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
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-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 17 Solutions
BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
Ch. 17.1 - Prob. 1MCCh. 17.1 - Prob. 2MCCh. 17.1 - What features were used to build the animal...Ch. 17.1 - Prob. 4MCCh. 17.1 - Prob. 5MCCh. 17.1 - What advantages does segmentation confer?Ch. 17.2 - Prob. 1MCCh. 17.2 - Prob. 2MCCh. 17.2 - Prob. 3MCCh. 17.2 - Prob. 4MC
Ch. 17.3 - Prob. 1MCCh. 17.3 - Prob. 2MCCh. 17.3 - Prob. 3MCCh. 17.3 - Prob. 4MCCh. 17.4 - Prob. 1MCCh. 17.4 - How does the body shape of a flatworm enhance gas...Ch. 17.4 - Prob. 3MCCh. 17.4 - Prob. 4MCCh. 17.5 - Prob. 1MCCh. 17.5 - Prob. 2MCCh. 17.5 - Prob. 3MCCh. 17.6 - What features do all annelids share?Ch. 17.6 - List examples of animals in each of the two...Ch. 17.6 - Prob. 3MCCh. 17.7 - Prob. 1MCCh. 17.7 - Prob. 2MCCh. 17.7 - Prob. 3MCCh. 17.7 - Prob. 4MCCh. 17.8 - Prob. 1MCCh. 17.8 - Prob. 2MCCh. 17.8 - Prob. 3MCCh. 17.8 - Prob. 4MCCh. 17.8 - Prob. 5MCCh. 17.8 - Prob. 6MCCh. 17.9 - What characteristics distinguish the echinoderms?Ch. 17.9 - Prob. 2MCCh. 17.9 - In what ways are echinoderms important?Ch. 17.10 - Prob. 1MCCh. 17.10 - Prob. 2MCCh. 17.10 - Prob. 3MCCh. 17.10 - Prob. 4MCCh. 17.11 - What is the relationship among tunicates,...Ch. 17.11 - Make a table comparing the features of each...Ch. 17.11 - Prob. 3MCCh. 17.11 - Prob. 4MCCh. 17.12 - Prob. 1MCCh. 17.12 - What can skeletal anatomy and DNA sequences in...Ch. 17.12 - What are the four groups of species in the hominin...Ch. 17.12 - Prob. 4MCCh. 17 - Following gastrulation, the cells that have folded...Ch. 17 - Prob. 2MCQCh. 17 - Prob. 3MCQCh. 17 - How is the body structure of an annelid different...Ch. 17 - Prob. 5MCQCh. 17 - Prob. 6MCQCh. 17 - Echinoderms have ____ symmetry as embryos and ____...Ch. 17 - Prob. 8MCQCh. 17 - Which of the following has pharyngeal slits at...Ch. 17 - Lobe-finned fishes are important because they a...Ch. 17 - To which of the following is a salamander most...Ch. 17 - How do reptiles and mammals differ from...Ch. 17 - Which of the following represents the correct...Ch. 17 - Primates share all of the following...Ch. 17 - DNA evidence suggests that modern humans a. share...Ch. 17 - Compare the nine major animal phyla in the order...Ch. 17 - Prob. 2WIOCh. 17 - Using the evolutionary trees in this chapter,...Ch. 17 - Prob. 4WIOCh. 17 - Prob. 5WIOCh. 17 - Prob. 6WIOCh. 17 - Prob. 7WIOCh. 17 - Create lists of animal phyla that a are...Ch. 17 - Prob. 9WIOCh. 17 - Draw from memory a phylogenetic tree that traces...Ch. 17 - Prob. 11WIOCh. 17 - List the evidence that biologists use to classify...Ch. 17 - Prob. 13WIOCh. 17 - Prob. 14WIOCh. 17 - Prob. 15WIOCh. 17 - Prob. 16WIOCh. 17 - Prob. 17WIOCh. 17 - Prob. 18WIOCh. 17 - In what ways has culture been an important factor...Ch. 17 - Prob. 20WIOCh. 17 - How do you predict a scientist would respond to a...Ch. 17 - Prob. 22WIOCh. 17 - Prob. 1SLCh. 17 - Prob. 2SLCh. 17 - Prob. 1PITCh. 17 - Prob. 2PITCh. 17 - Draw a concept map that summarizes the chordates,...Ch. 17 - Add tunicates, hagfishes, birds, monotremes,...Ch. 17 - Other than the ones pictured, give an example of a...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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