ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
4th Edition
ISBN: 9781265061173
Author: McKinley
Publisher: MCG CUSTOM
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Textbook Question
Chapter 17.6, Problem 14WDYL
How does down-regulation of cellular receptors change responsiveness to a given hormone?
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 17 Solutions
ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
Ch. 17.1 - Prob. 1WDYLCh. 17.1 - Prob. 2WDYLCh. 17.1 - Prob. 3WDYLCh. 17.2 - Prob. 4WDYLCh. 17.2 - Prob. 5WDYLCh. 17.3 - Identify which of the following hormone categories...Ch. 17.3 - What two events or processes associated with a...Ch. 17.3 - Prob. 8WDYLCh. 17.4 - Why are carrier proteins necessary for...Ch. 17.4 - What is the added benefit of a carrier protein?
Ch. 17.4 - What is the relationship of hormone synthesis to...Ch. 17.5 - Where are lipid-soluble hormone receptors located?...Ch. 17.5 - Prob. 13WDYLCh. 17.6 - How does down-regulation of cellular receptors...Ch. 17.6 - What effects are seen when hormones act...Ch. 17.7 - What is the anatomic connection between the...Ch. 17.7 - Prob. 17WDYLCh. 17.7 - Prob. 18WDYLCh. 17.7 - Prob. 19WDYLCh. 17.7 - Prob. 20WDYLCh. 17.8 - Prob. 21WDYLCh. 17.8 - What is the relationship of TRH, TSH, and TH in...Ch. 17.8 - What are the primary target organs/issues of TH?...Ch. 17.8 - Prob. 24WDYLCh. 17.9 - Prob. 25WDYLCh. 17.9 - Prob. 26WDYLCh. 17.9 - Prob. 27WDYLCh. 17.10 - Prob. 28WDYLCh. 17.10 - Prob. 29WDYLCh. 17.10 - Prob. 30WDYLCh. 17.10 - Prob. 31WDYLCh. 17.11 - Prob. 32WDYLCh. 17.11 - Prob. 33WDYLCh. 17.11 - Prob. 34WDYLCh. 17.11 - Prob. 35WDYLCh. 17.12 - Prob. 36WDYLCh. 17 - Prob. 1DYKBCh. 17 - This hormones primary function is to regulate...Ch. 17 - Which of the following are components of...Ch. 17 - A hormone released from the anterior pituitary is...Ch. 17 - Prob. 5DYKBCh. 17 - Prob. 6DYKBCh. 17 - Glucagon has an __________ effect to insulin on...Ch. 17 - Glucocorticoids (e.g., cortisol) are produced in...Ch. 17 - Thyroid-stimulating hormone stimulates the a....Ch. 17 - Prob. 10DYKBCh. 17 - Prob. 11DYKBCh. 17 - Prob. 12DYKBCh. 17 - Explain the three mechanisms used to stimulate...Ch. 17 - Identify the three chemical classes of hormones,...Ch. 17 - Describe how local hormones differ from...Ch. 17 - Explain the function of carrier proteins in...Ch. 17 - Describe how water-soluble hormones interact with...Ch. 17 - Explain how the hypothalamus oversees and controls...Ch. 17 - Explain how the hypothalamus oversees and controls...Ch. 17 - Discuss the homeostatic system involving insulin.Ch. 17 - George is a 43-year-old construction worker who...Ch. 17 - What is the best diagnostic test to determine if...Ch. 17 - Jelena is late for work and is rushing to get out...Ch. 17 - Blood samples from a young woman named Michelle...Ch. 17 - Stephen is taking a new weight-loss supplement...Ch. 17 - Prob. 1CSLCh. 17 - Prob. 2CSLCh. 17 - Henry is a well-informed patient who is interested...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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