PRESCOTT'S MICROBILOGY
11th Edition
ISBN: 9781264075515
Author: WILLEY
Publisher: MCG
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Textbook Question
Chapter 17.2, Problem 1CC
Briefly describe the polymerase chain reaction. What is the difference between a step and a cycle?
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Briefly describe the polymerase chain reaction. What is the difference between a step and a cycle?
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Chapter 17 Solutions
PRESCOTT'S MICROBILOGY
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MICh. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC
Ch. 17.1 - Prob. 4CCCh. 17.1 - Prob. 5CCCh. 17.2 - Why, after three cycles, are the vast majority of...Ch. 17.2 - Briefly describe the polymerase chain reaction....Ch. 17.2 - Why is PCR used to detect infectious agents that...Ch. 17.2 - How would you use PCR to measure the concentration...Ch. 17.2 - Why is it possible to visualize a PCR product on...Ch. 17.2 - Prob. 5CCCh. 17.3 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.4 - What special considerations are necessary if one...Ch. 17.4 - Prob. 1CCCh. 17.4 - Prob. 2CCCh. 17.4 - Prob. 3CCCh. 17.4 - You are studying chemotaxis proteins in a newly...Ch. 17.5 - Prob. 1MICh. 17.5 - Prob. 1CCCh. 17.5 - Prob. 2CCCh. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - Prob. 1RCCh. 17 - Prob. 2RCCh. 17 - Prob. 3RCCh. 17 - Prob. 4RCCh. 17 - Prob. 5RCCh. 17 - Prob. 6RCCh. 17 - Prob. 1ALCh. 17 - Prob. 2ALCh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5ALCh. 17 - Prob. 6ALCh. 17 - Prob. 7AL
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- How many different temperatures are used in each cycle of the polymerase chain reaction?arrow_forwardThe synthetic unit of the polymerase chain reaction is the replica. True or false?arrow_forwardAssume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single DNA fragment will there be after the reaction has been run for six hours?arrow_forward
- What is the difference between terminal deoxynucleotidyl transferase from other polymerases?arrow_forwardwhat are some Limitations/sources of error with suitable rationalization that may occur during the polymerase chain reaction?arrow_forwardWhat difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?arrow_forward
- Which of the followings statements are true about DNA polymerase? 1.) It can only go in one direction, meaning the lagging strand can't be synthesized continuously. 2.) It cannot start a DNA strand from scratch, so another enzyme is needed to create "primers" as a starting point. 3.) It cannot copy epigenetic marks (such as methyl groups) on its own; these must be "copied" onto the daughter DNA strand by other enzymes after DNA replication. 4.) All of the abovearrow_forwardDescribe the main steps in using the polymerase chain reaction to amplify a small amount of DNA.arrow_forwardEach cycle of the polymerase chain reaction consists of a shift between three different temperatures (94 C, 60 C, and 72 C), explain what occurs at each of those temperatures.arrow_forward
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