PRESCOTT'S MICROBILOGY
PRESCOTT'S MICROBILOGY
11th Edition
ISBN: 9781264075515
Author: WILLEY
Publisher: MCG
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Chapter 17.1, Problem 4CC
Summary Introduction

To describe: The process of antibiotic resistance selection followed by blue-white screening of the transformant cells.

Introduction: Selective markers are the genes which infer specific traits to a bacterium or cell and serves to facilitate artificial selection.

Summary Introduction

To determine: The importance of the consecutive use of both antibiotic and blue-white colony screening techniques.

Introduction: Selective markers are used to separate the recombinant cells from the non-recombinant cells. The selective markers used are antibiotic resistance genes and the lac Za gene..

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The shape of radishes may be long (SL/SL), oval (SL/SS), or round (SS/SS), and the color of radishes may be red (CR/CR), purple (CR/CW) or white (CW/CW).  If a long, red radish plant is crossed with a round, white plant, what will be the appearance of the F1 and F2 generations?
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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Chapter 17 Solutions

PRESCOTT'S MICROBILOGY

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