PRESCOTT'S MICROBILOGY
PRESCOTT'S MICROBILOGY
11th Edition
ISBN: 9781264075515
Author: WILLEY
Publisher: MCG
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Chapter 17.1, Problem 4CC
Summary Introduction

To describe: The process of antibiotic resistance selection followed by blue-white screening of the transformant cells.

Introduction: Selective markers are the genes which infer specific traits to a bacterium or cell and serves to facilitate artificial selection.

Summary Introduction

To determine: The importance of the consecutive use of both antibiotic and blue-white colony screening techniques.

Introduction: Selective markers are used to separate the recombinant cells from the non-recombinant cells. The selective markers used are antibiotic resistance genes and the lac Za gene..

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Explain down bellow what happens to the cell in pictures not in words: Decreased pH in mitochondria Increased ATP Decreased pH in cytosol Increased hydrolysis Decreasing glycogen and triglycerides Increased MAP kinase activity Poor ion transport → For each one:→ What normally happens?→ What is wrong now?→ How does it mess up the cell?
1.) Community Diversity: The brown and orange line represent two different plant communities. a. Which color represents the community with a higher species richness? b. Which color represents the community with a higher species evenness? Relative abundance 0.1 0.04 0.001 2 4 6 8 10 12 14 16 18 20 22 24 Rank abundance c. What is the maximum value of the Simpson's diversity index (remember, Simpson's index is D = p², Simpson's diversity index is 1-D)? d. If the Simpson's diversity index equals 1, what does that mean about the number of species and their relative abundance within community being assessed?
1.) Community Diversity: The brown and orange line represent two different plant communities. a. Which color represents the community with a higher species richness? b. Which color represents the community with a higher species evenness? Relative abundance 0.1 0.04 0.001 2 4 6 8 10 12 14 16 18 20 22 24 Rank abundance c. What is the maximum value of the Simpson's diversity index (remember, Simpson's index is D = p², Simpson's diversity index is 1-D)? d. If the Simpson's diversity index equals 1, what does that mean about the number of species and their relative abundance within community being assessed?

Chapter 17 Solutions

PRESCOTT'S MICROBILOGY

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