MAKE CONNECTIONS → In a research artide about alkaptonuria published in 1902, Garrod suggested that humans inherit two "characters" (alleles) for a particular enzyme and that both parents must contribute a faulty version for the offspring to have alkaptonuria. Today, would this disorder be called dominant or recessive? (See Concept 14.4.)
1. What is a promoter? Is it located at the upstream or downstream end of a transcription unit?
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- E30. An electrophoretic mobility shift assay can be used to study the binding of proteins to a segment of DNA. In the experiment shown here, an EMSA was used to examine the requirements for the bind- ing of RNA polymerase II (from eukaryotic cells) to the promoter of a protein-encoding gene. The assembly of general transcription factors and RNA polymerase II at the core promoter is described in Chapter 12 (Figure 12.14). In this experiment, the segment of DNA containing a promoter sequence was 1100 bp in length. The fragment was mixed with various combinations of proteins and then subjected to an EMSA. Lane 1: No proteins added Lane 2: TFID Lane 3: TFIIB Lane 4: RNA polymerase I| Lane 5: TFID + TFIIB Lane 6: TFID + RNA 1 2 4 5 6 polymerase II| Lane 7: TFIID + TFIIB + RNA polymerase I| 1100 bp Explain which proteins (TFIID, TFIIB, or RNA polymerase II) are able to bind to this DNA fragment by themselves. Which transcrip- tion factors (i.e., TFIID or TFIIB) are needed for the binding of…arrow_forwardhelparrow_forwardE27. A cloned gene fragment contains a regulatory element that is recog- nized by a regulatory transcription factor. Previous experiments have shown that the presence of a hormone results in transcriptional acti- vation by this transcription factor. To study this effect, you conduct a electrophoretic mobility shift assay and obtain the following results: Tube: 1 2 3 Transcription factor: Hormone: Explain the action of the hormone.arrow_forward
- 23arrow_forwardD,E,Farrow_forwardThe enormous complexity in initiating eukaryotic gene transcription seems at first glance to be wasteful. What is the most plausible reason for requiring so many different proteins to assemble on a large array of DNA sequences in order to initiate transcription? O The use of large numbers of proteins and regulatory sequences in DNA opens many possibilities for sophisticated combinatorial gene regulation O The need to open chromatin for transcription demands a much larger number of proteins and regulatory sequences in DNA O The use of large numbers of proteins and regulatory sequences in DNA is essential for alternative splicing of hnRNA in eukaryotes O The larger number of eukaryotic genes demands a correspondingly larger transcription apparatus than that of prokaryotes The larger size of eukaryotic cells demands a correspondingly larger transcription apparatus than that of prokaryotesarrow_forward
- 24arrow_forwardⒸ Macmillan Learning Classify the given examples of prokaryotic gene expression as positive or negative gene regulation. 54 $ R In the presence of excess tryptophan, a repressor protein binds the operator of the trp operon and prevents the operon from being transcribed. In the absence of lactose, the lacR repressor protein binds the lac operon. F4 Positive gene regulation In the presence of the sugar arabinose, an activator protein binds the promoter of the genes responsible for processing arabinose and induces their transcription. In the presence of iron, the dtxR repressor protein binds DNA, and the gene that encodes for the diphtheria toxin is not expressed. % 5 In the presence of lactose and low glucose, the lac operon expressed 20-fold higher than in the absence of lactose. T F5 < 6 MacBook Air MA F6 Answer Bank & 7 F7 Y U * 8 DII F8 Negative gene regulation 1 ( 9 F9 O ) - C J F10 | ! LICarrow_forwardpls help me to do these my activity and answer properlyarrow_forward
- Hơ many mismatches would you expect to find between this sequence and the reference sequence? I know the answer is 3, can you please explain why?arrow_forwardPlz do explain.Thanks Question:- Many types of breast cancer have chromosomal translocation mutations. What scenario best describes, what occurs during this type of mutation, causing cells to proliferate abnormally? Chromosomal translocations may place the gene downstream near the promoter region, therefore causing over-expression of the gene The gene may be placed in the transcription start site, downstream of the gene, initiating transcription by recruiting polymerase II Translocation mutations will initiate the transcription of mRNA in the cytoplasm of the cell catalyzing protein synthesis. Chromosomal translocations can sometimes place a gene under the control of a powerful enhancer, upstream.arrow_forwardPlz asaparrow_forward
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