BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
12th Edition
ISBN: 9781264058167
Author: Raven
Publisher: MCG CUSTOM
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Chapter 17.1, Problem 1LO
Summary Introduction

To describe: The way in which the ligases and restriction endonucleases are used to make recombinant DNA.

Introduction: Deoxyribonucleic acid (DNA) is the hereditary material present in humans and almost all organisms. It contains the genetic information required for the development and functioning of living organisms. A single DNA molecule formed using DNA from two different sources is called as the recombinant DNA.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’

Chapter 17 Solutions

BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<

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