The application of FISH in obtaining the telomeric chromosomes in the G 2 phase of the cell cycle. Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

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Answer 1

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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Answer 2

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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Answer 3

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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Answer 4

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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Answer 5

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Answer 6

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Answer 7

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Answer 8

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.