Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Chapter 17, Problem 6TYU
Summary Introduction
To draw:
The replicative cycle of a virus within a single-stranded genome that can function as mRNA.
Introduction:
Viruses are microbes that divide and increase their number using the replicative machinery of the host. Mostly, they have single stranded RNA as their genetic material but some viruses have double-stranded DNA as their genetic material.
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The diagrams below represent nucleic acid genomic material and a finished product after
the viral polymerase acted on the genomic material. Name the virus (either family, genus,
or particular virus is acceptable) that these diagrams represent.
A is
B is
C is
Dis
Genomic material
Product
+ sense RNA
A 5' VPB
-sense RNA
3'
VPg 5'
-sense RNA
5' cap
Derived from infected cell
B
3'
5'
AAAAA(A) 3
+ sense RNA
C
+ sense RNA
-sense RNA
+sense RNA
5'
3'
Assume you isolate a single stranded (+) RNA virus. When you examine the proteins in the virus, you find that it does NOT contain replicase enzymes within its capsid. Which of the following is true?
This virus must have a gene that encodes replicase.
This virus will not be able to enter a host cell.
Its genome cannot be translated (the process of translation) by the host cell ribosomes.
A DNA copy of the viral genome has to be made before viral genes are expressed.
This virus must lack surface antigens.
Viruses are made up of the same biomolecules as cellular organisms (DNA, RNA, proteins, lipids, etc.), which are synthesized through enzymatic dehydration reactions from building blocks and disassembled by enzymatic hydrolysis. They infect cells and replicate themselves using resources from the host cells. Scientists discovered a previously unknown strain of virus in the tropical Pacific Ocean and it can infect higher-order eukaryotes. Initial analysis revealed that the virus is mainly composed of nucleic acids, proteins, and lipids. Chemical analysis after complete hydrolysis revealed the following building blocks (by weight):
Chemical components
Nucleosides
Amino acids
Lipids, phosphates, and carbohydrates
Percentage by weight
20
30
50
Analysis of percentages of nucleosides yielded the following results (by molar ratio):
Nucleosides
A
G
T
C
U
Percentage by mole
19
29
19
29
4
Which of the following events mostly involves hydrolysis reaction after the virus infects…
Chapter 17 Solutions
Campbell Biology in Focus
Ch. 17.1 - Compare the structures of tobacco mosaic virus and...Ch. 17.1 - Prob. 2CCCh. 17.2 - Compare the effect on the host cell of a ly1ic...Ch. 17.2 - MAKE CONNECTIONS The RNA virus in Figure 17.7 has...Ch. 17.2 - Why is HIV called a retrovirus?Ch. 17.2 - MAKE CONNECTIONS Compare the CRISPR system to the...Ch. 17.3 - Describe two ways in which a preexisting virus...Ch. 17.3 - Contrast horizontal and vertical transmission of...Ch. 17.3 - Prob. 3CCCh. 17 - which of me following characteristics. structures....
Ch. 17 - Prob. 2TYUCh. 17 - A human pandemic is A. a viral disease that...Ch. 17 - Prob. 4TYUCh. 17 - RNA viruses require their own supply of certain...Ch. 17 - Prob. 6TYUCh. 17 - Prob. 7TYUCh. 17 - Prob. 8TYUCh. 17 - FOCUS ON ORGANIZATION While viruses are considered...Ch. 17 - SYNTHESIZE YOUR KNOWLEDGE Oseltamivir (Tamiflu),...
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- The dUTPase enzyme is used by the African swine fever virus to repair its own DNA, as the cells the virus infects in swine do not express the dUTPase protein. Which of the following is a reasonable explanation for the role of the dUTPase in the virus lifecycle? Explain your choice in 25 words or less. The dUTPase is necessary to remove dUTP from the viral genome, as uracil should not be present in DNA The dUTPase is necessary to remove dUTP from the viral genome, as uridine cannot correctly base pair with adenine-containing bases The dUTPase is necessary to remove dUTP from the viral genome, as uridine has the wrong sugar component for building DNA More than one of the above answers is correct None of the above answers is correctarrow_forwardDraw a generic (+)RNA virus replication cycle. For viruses, (+) RNA indicates the genomic RNA can be translated directly. How would this differ from the system you drew in #1? Draw a generic (+) RNA virus replication cycle. Indicate which proteins need to be present for RNA replication and translation. Where would they act? Indicate what sequences or modifications would need to be present on the virus RNA for RNA replication and translation. How might a virus differentiate between RNA to be put into progeny virus particles and RNA to be translated into protein?arrow_forwardSome retroviruses that have a wide host range have been developed for use in treatment of diseases or syndromes. These viruses do not result in a productive infection because they lack critical genes for viral replication, but they do carry DNA sequences that allow them to be packaged into infectious particles. Once in the host cell the viral DNA is released and integrates into the cell's genome. The advantage to this strategy is that the delivery of the DNA is very efficient. What is one possible problem of using a virus that can integrate into the human nuclear genome? Multiple Choice The virus carries genes that will interfere with host cell activities. The viral DNA sequences could still cause a disease. Since the viral DNA sequences no longer contain essential genes for viral replication the host cell may not be able to replicate either. The integration could disrupt an essential gene or genes.arrow_forward
- The RNA genomes of some RNA viruses resemble the messenger RNA (mRNA) of their eukaryotic hosts. What advantage would an RNA virus gain by having this type of genome?arrow_forwardViruses are made up of the same biomolecules as cellular organisms (DNA, RNA, proteins, lipids, etc.). They infect cells and replicate themselves using resources from the host cells. Scientists discovered a previously unknown strain of virus in the tropical Pacific Ocean and it can infect higher-order eukaryotes. Initial analysis revealed that the virus is mainly composed of nucleic acids, proteins, and lipids. Chemical analysis after complete hydrolysis revealed the following building blocks (by weight): Chemical components Nucleosides Amino acids Lipids, phosphates, and carbohydrates Percentage by weight 20 30 50 Analysis of percentages of nucleosides yielded the following results (by molar ratio): Nucleosides A G T C U Percentage by mole 19 29 19 29 4 Researchers determined that the lipids found in the virus form a bilayer membrane surrounding the virus, and are obtained from the membrane of the host cell during virus maturation and exit from host cell. Further…arrow_forwardFigure 1 above shows that remdesivir “mimics” an important component of RNA replication. Which component of RNA replication has a structure similar to that of remdesivir?arrow_forward
- Viruses with negative sense RNA genomes typically, make proteins by: (Ignore retroviruses, and the unusual characteristics of coronaviruses) Translation of short RNA transcripts generated by RDRP Generating a DNA copy, which is then transcribed by host RNA polymerase. Translation of the viral genome by host ribosomes. Production of a polyprotein, which must be cleaved into smaller proteins. Generating a negative sense genome copy, which is then translated by host ribosomes.arrow_forwardYou are studying RNA viruses and have discovered one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don't know if it is positive or negative stranded. Your lab-mate says, "Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome." You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate's suggestion?arrow_forwardAs a general rule, viruses are not considered to be alive based on several reasons. One such reason is that some viruses use RNA as their genetic material instead of DNA. Which of the five biological themes does this violate? Briefly explain why.arrow_forward
- Name a nucleoside analog. Explain how that nucleoside analog works. Which viruses are effectively treated by that nucleoside analog?arrow_forwardSyn5 is a virus that infects photosynthetic bacteria belonging to the genus Synechococcus. The Syn5 virion is icosahedral (660 Å in diameter) with a short tail and an appendage called a horn. The horn is located on a vertex directly opposite the tail. Syn5 follows the typical five steps observed for most viruses when they multiply, and progeny viruses are released when the host lyses. The assembly of Syn5 virions involves several proteins, including capsid proteins, scaffolding proteins, a portal protein, a terminase, tail proteins, and horn proteins. To better understand the assembly of Syn5 virions, scientists used a type of microscopy called Zernike phase contrast electron cryotomography. This type of microscopy improves the contrast typically seen for conventional cryotomography. In their studies, the scientists observed several different developing virus particles in infected cells. The particles were: (1) 660 Å icosahedron containing DNA and having a tail; (2) 660 Å icosahedron…arrow_forwardIf you must create a drug or prescribe a healthy food or drink to target the Covid virus, what part of the virus will you target? Why? Show the functional groups that will interact with the specific part of Covid.arrow_forward
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What Is A Virus ? ; Author: Peekaboo Kidz;https://www.youtube.com/watch?v=YS7vsBgWszI;License: Standard YouTube License, CC-BY