Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 17, Problem 5CHI
Summary Introduction
The polymerase chain reaction (PCR) was designed by Kary Mullis in the year 1980. This technique is used to amplify the small amount of specific DNA sequences. Billions of copies of specific DNA from a complex mixture can be rapidly synthesized by the PCR method. This technique has numerous applications. It is used in obtaining the genes for cloning. It is also used in medical diagnosis, clinical, research, and
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Chapter 17 Solutions
Prescott's Microbiology
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Why might two DNA fragments inadvertently be...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - If a linear piece of DNA is cut with a restriction...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - You want to visualize a digested plasmid that...Ch. 17.1 - What is the purpose of Southern blotting? How is a...Ch. 17.2 - Why, after three cycles, are the vast majority of...
Ch. 17.3 - Which plasmid is a shuttle vector? Why?Ch. 17.3 - What would you conclude if you obtained only blue...Ch. 17.3 - In what ways does the BAC shown here differ from...Ch. 17.3 - Briefly describe the polymerase chain reaction....Ch. 17.3 - Why is PCR used to detect infectious agents that...Ch. 17.3 - How would you use PCR to measure the concentration...Ch. 17.3 - Why is it possible to visualize a PCR product on...Ch. 17.3 - You want to clone a 6,000 bp DNA fragment in E...Ch. 17.5 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.6 - What special considerations are necessary if one...Ch. 17.6 - Prob. 1RIACh. 17.6 - Explain the selection for antibiotic resistance...Ch. 17.6 - Prob. 3RIACh. 17.6 - Prob. 4RIACh. 17.6 - Prob. 5RIACh. 17.6 - You are studying chemotaxis proteins in a newly...Ch. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - You are performing a PCR to amplify a gene...Ch. 17 - Prob. 2CHICh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5CHICh. 17 - Zymomonas mobilis is a Gram-negative bacterium...
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
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