BIOLOGY 12E CONNECT ACCESS CARD
12th Edition
ISBN: 9781264938513
Author: Raven
Publisher: MCG
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Chapter 17, Problem 3U
Summary Introduction
Introduction:
PCR stands for Polymerase chain reaction. It is a technique that is used to amplify a single fragment of DNA to generate large copies of that particular fragment of DNA. This technique was developed by Kary Mullis in 1983.
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If a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10 cycles?
a. About 100
b. About 1000
c. About 10,000
d. About 1010
Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
Would it be possible to use human polymerase for the PCR reaction?
a.
No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur.
b.
No, because human polymerase cannot be extracted from cells to use in a lab setting.
c.
Yes, because we are using human DNA as the template DNA.
d.
Yes, because human polymerase can add bases to a template strand without a primer.
Chapter 17 Solutions
BIOLOGY 12E CONNECT ACCESS CARD
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- A prokaryotic genome is about 4 million bp in length. About howmany genes would you expect it to contain?a. 400b. 4000c. 40,000d. 400,000arrow_forwardGenetic engineering is the direct modification of an organisms’ DNA using biotechnology. What are the different techniques in genetic engineering? a. Inoculate, Gel electrophoresis, Budding and Cloning b. Grafting, Gene splicing, Budding and Inbreeding c. Cloning, Gene splicing, Gel electrophoresis and Hybridization d. Gene splicing, Grafting, Hybridization and Cloningarrow_forwardWhich type of genomics studies the transcripts and proteins expressed by a genome? a. Comparative genomics b. Structural genomics c. Proteogenomics d. Functional genomicsarrow_forward
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