The application of FISH in obtaining the telomeric chromosomes in the G 2 phase of the cell cycle. Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

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Answer 1

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.) Data Table 1Stage Totals PercentInterphase Mitosis: Prophase Metaphase Anaphase Telophase Cytokinesis Totals 100 100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…

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Answer 2

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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Answer 3

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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Answer 4

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

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Answer 5

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Answer 6

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Answer 7

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Answer 8

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.