EP CONNECT ONLINE ACCESS FOR BIOLOGY
20th Edition
ISBN: 9781260494655
Author: Raven
Publisher: MCG COURSE
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Chapter 17, Problem 6U
The Ti plasmid of Agrobacterium usually induces tumors called galls on infected plants. When Agrobacterium is used to make transgenic plants, why does no gall form?
a. The introduced transgene blocks the action of the gall-inducing genes.
b. The gall-inducing genes are removed and replaced with the transgene.
c. The Ti plasmid is removed from Agrobacterium used to make transgenic plants.
d. The Ti plasmid is mutated by PCR so that it cannot replicate in infected plants.
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Chapter 17 Solutions
EP CONNECT ONLINE ACCESS FOR BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- Agrobacterium tumefaciens is a bacterium that infects a wide range of broad-leaved plants. During infection, a plasmid called Ti is transferred into the plant cells, where that plasmid DNA is integrated into the plant’s genome. Some of the genes encoded by this plasmid are expressed by host cell machinery in the plant cell. These gene products produce plant hormones which stimulate cell division in the infected cells, causing the development of a plant tumor which provides an environment for that bacterium to grow. You are studying a novel isolate of A. tumefaciens and want to determine which of the genes on the Ti plasmid are expressed in the bacterium, and which are expressed in the plant. You start by sequencing the Ti plasmid. a) Considering the DNA sequence of Ti, describe two specific genetic features that would help you to differentiate whether a given gene is likely expressed in the plant or bacterium. b) You collect plant tumor tissue, containing both bacterial and plant…arrow_forwardPlasmids are important for recombining DNA. B.) What are the requirments of an expression vector.arrow_forwardPrimary cultures and limited cell lines have a low plating efficiency, which will influence cell survival rates. Make suggestions about how to increase the cloning procedure's plating efficiency.arrow_forward
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- Transgenic crops often require fewer herbicides and insecticides than conventional crops. In that respect, they could be considered environmentally friendly. Use the Internet to research the question of why some environmental groups oppose transgenic technology.arrow_forwardUsing nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid.What are restriction length polymorphisms, and how are they used?arrow_forwardIn a molecular biology laboratory, a student obtained competent E. coli cells and used a common transformation procedure to induce the uptake of plasmid DNA with a gene for resistance to the antibiotic kanamycin. The results below were obtained. Plate I. LB agar +kan plasmid Plate II. LB agar with kanamycin +kan plasmid Plate IV. Plate II. LB agar no plasmid added LB agar with kanamycin no plasmid added On which petri dish do only transformed cells grow? b. Plate I| a. Plate I c. Plate III d. Plate IV Which of the plates is used as a control to show that non-transformed E. coli will not grow in the presence of kanamycin? a. Plate I b. Plate II c. Plate II d. Plate IV If a student wants to verify that transformation has occurred, which of the following procedures should s/he use? a. Spread cells from Plate I onto a plate with LB agar, incubate. b. Spread cells from Plate Il onto a plate with LB agar, incubate. c. Repeat the initial spread of-kanR cells onto plate IV to eliminate…arrow_forward
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