Chapter 17, Problem 16PIAT

Summary Introduction

To analyze:

Setting up an experiment to determine whether the α-amanitin toxin affects protein synthesis.

Introduction:

The α-amanitin is an 8-amino acid long cyclic peptide, which acts as a toxin if ingested. It is found in the Amanita phalloides (death cap mushroom). The primary cause of death from α-amanitin poisoning is liver failure. Suppose a physician informs you that the liver cells die because their rate of protein production falls below a level needed to maintain active metabolism. Given that α-amanitin is an inhibitor of transcription, one wonders if this information is correct.