Biology: The Dynamic Science
Biology: The Dynamic Science
4th Edition
ISBN: 9781337246422
Author: Peter J. Russell; Paul E. Hertz; Beverly McMillan
Publisher: Cengage Learning US
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Chapter 16.4, Problem 1SB
Summary Introduction

To review:

The process of differentiation and determination, along with the mechanism of their control.

Introduction:

All the cells that are nucleated have the same set of genes. The functional as well as structural variations in the cells are known to be determined not only by the presence or absence of some specific gene but also through the differences in the organization of gene expression, which regulates the synthesis of cell type-specific groups of proteins.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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Cell Differentiation | Genetics | Biology | FuseSchool; Author: FuseSchool - Global Education;https://www.youtube.com/watch?v=gwAz_BtVuLA;License: Standard YouTube License, CC-BY