Biology
12th Edition
ISBN: 9780134813448
Author: Audesirk, Teresa, Gerald, Byers, Bruce E.
Publisher: Pearson,
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Chapter 16.2, Problem 1TC
If it were true that mutations do occur in response to the presence of antibiotics. how would the result of this experiment have differed from the actual result?
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When the Oxford team of Ernst Chain and Nor- man Heatley had laboriously collected their first two grams of penicillin (probably no more than 2% pure!), Chain injected two normal mice with 1 g each of this preparation, and waited to see what would happen. The mice survived with no apparent ill effects. Their boss, Howard Florey, was furious at what he saw as a waste of good antibiotic. Why was this experiment important?
Some bacteria may have higher mutation rates than others following exposure to UV light. Discuss a reason why this might be the case. What experiments could you do to determine whether this is a likely possibility?
All of the following apply to Luria and Delbrűck’s mutation theory as tested using E. Coli and T1 phage EXCEPT:
A. It was performed using liquid bacterial cultures
B. It involved looking for T1-resistant bacteria as an end measure
C. It was supported by the finding that the number of final cultures that were T1 phage-resistant were constant from experiment to experiment
D. It rejected the possibility of mutations occurring only in response to a T1 phage challenge
E. It supported the concept of spontaneous mutation rates
Chapter 16 Solutions
Biology
Ch. 16.1 - define evolution in terms of concepts from...Ch. 16.1 - define equilibrium population and describe the...Ch. 16.2 - If it were true that mutations do occur in...Ch. 16.2 - Explain how the distribution of genotypes in...Ch. 16.2 - A flu vaccination stimulates your immune system to...Ch. 16.2 - Prob. 3TCCh. 16.2 - If a population grows large again after a...Ch. 16.2 - Prob. 5TCCh. 16.2 - Evolution of a Menace The mutant alleles that...Ch. 16.2 - describe how mutation, gene flow, genetic drift,...
Ch. 16.3 - A team of phys clans treated four patients with...Ch. 16.3 - If we studied a population of bighorn sheep and...Ch. 16.3 - When selection is directional, is there any limit...Ch. 16.3 - describe why selection of phenotypes can affect...Ch. 16.3 - explain how competition and predation influence...Ch. 16.3 - Prob. 3CYLCh. 16.3 - compare and contrast directional selection,...Ch. 16.3 - Microbiologists have discovered that alleles...Ch. 16 - The alleles responsible for antibiotic resistance...Ch. 16 - Stabilizing selection on a trait tends to a. make...Ch. 16 - An adaptation is a. any trait that arises from a...Ch. 16 - Which of the following statements about mutations...Ch. 16 - Genetic drift occurs a. when different phenotypes...Ch. 16 - The ______ provides a simple mathematical model...Ch. 16 - Different versions of the same gene are called...Ch. 16 - An organisms ______ refers to the specific alleles...Ch. 16 - A random form of evolution is called ________....Ch. 16 - Competition is most Intense between members of...Ch. 16 - The evolutionary fitness of an organism is...Ch. 16 - What is a gene pool? How would you determine the...Ch. 16 - Define equilibrium population. Outline the...Ch. 16 - How does population size affect the likelihood of...Ch. 16 - If you measured the allele frequencies of a gene...Ch. 16 - People like to say that you cant prove a negative....Ch. 16 - Describe the three ways in which natural selection...Ch. 16 - What is sexual selection? How is sexual selection...Ch. 16 - In North America, the average height of adult...Ch. 16 - By the 1940s, the whooping crane population had...
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- All questions a) What is the hypothesis of Griffith’s experiment? b) Why did mouse No. 3 survived? c) Why did mouse No. 4 died? d) If Griffith had injected a fifth mouse with a combination of heat-killed R strain bacteria and heat-killed S strain bacteria, would the mouse have died? Give your reason.arrow_forwardHershey and Chase used radioactive components to tag protein and DNA before infecting bacteriophage. What was the most important concept demonstrated by Hershey-Chase experiments? Explain the experiment in detail.arrow_forwardIn Kornberg’s initial experiments, it was rumored that he grew E. coli in Anheuser-Busch beer vats. (Kornberg was working at Washington University in St. Louis.) Why do you think this might have been helpful to the experiment?arrow_forward
- One experiment that showed the DNA carried genetic information was the "Transformation" experiment by Avery, McCarty, and MacLeod where they injected mice with non-pathogenetic bacteria that had been incubated in the remains of pathogenic bacteria. What did they find when they incubated non-pathogenic bacteria in just the protein from the pathogenic bacteria? In this case there was no transformation They found that the non-pathogenic bacteria were transformed into pathogenic bacteria In this case the mice were sickened but did not die They found that some of the protein could cause transformation but other proteins could notarrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardYou perform an Ames test to determine which compound X causes mutations or not. You grow an auxotrophic strain of bacteria on three plates; one with no compound X, one with just compound X and one with compound X mixed with liver enzymes. You find many more colonies on the plates with compound X and with compound X plus liver enzymes compared to the control plate. Which of the following describes the best interpretation of the results? Compound X causes mutations on it's own but is safe after exposure to liver enzymes. Compound X is not mutagenic. Compound X is mutagenic regardless of whether it has been processed by liver enzymes. Compound X is mutagenic only after being treated with liver enzymes.arrow_forward
- C. Anagnostopoulos and I. P. Crawford isolated and studied a series of mutations that affected several steps in the biosynthetic pathway leading to tryptophan in the bacterium Bacillus subtilis . Seven of the strains that they used in their study are listed here, along with the mutation found in each strain.Strain Mutation T3 T− 168 I− 168PT I− TI I− TII I− T8 A− H25 H− To map the genes for tryptophan synthesis, they carried out a series of transformation experiments on strains having different mutations and determined the percentage of recombinants among the transformed bacteria. Their results were as follows:(refer picture) On the basis of these two-point crosses, determine the order of the genes and the distances between them. Where more than one cross was completed for a pair of genes, average the recombination rates from the different crosses. Draw a map of the…arrow_forwardHow might a bacteriophage be used to treat bacterial infections in humans?arrow_forwardConsider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.arrow_forward
- Chemical mutagens often cause oxidation or deamination of DNA bases. This can lead to cancer by causing: A) Activation of replication. B) Activation of transcription. C) Non Watson-Crick base pairing. OD) Changes to cytochrome P450 enzymes. OE) Activation of translation.arrow_forwardDescribe the experiment done by Frederick Griffith in 1928, where the non-lethal (rough) strain of Streptococcus pneumoniae bacteria killed experimental mice when mixed with the heat-killed smooth strain. Discuss what may have happened to the rough strain after the heat-killed smooth strain was introduced and why you think the rough strain killed the mice. What would be the conclusion and significance of this study and its finding?arrow_forwardIn Experiment I, you established that beneficial mutations are rare. In Experiment II, you showed that the environment does not affect the probability of a beneficial mutation occurring. Knowing this, why then are mutations responsible for antibiotic resistance in bacteria so common?arrow_forward
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