The state of rigor should be explained in molecular terms. Introduction: In muscle contraction, myosin filaments are being slide along the actin filaments. According to Albert Szent’s experiments, it is proven that the ATP hydrolysis and association and dissociation of actin and myosin complex are coupled. Energy released by ATP hydrolysis is used to power the muscle contraction.

Question

Please help determine the standard curve for my Kinase Activity in Excel Spreadsheet. Link: https://mnscu-my.sharepoint.com/personal/vi2163ss_go_minnstate_edu/_layouts/15/Doc.aspx?sourcedoc=%7B958f5aee-aabd-45d7-9f7e-380002892ee0%7D&action=default&slrid=9b178ea1-b025-8000-6e3f-1cbfb0aaef90&originalPath=aHR0cHM6Ly9tbnNjdS1teS5zaGFyZXBvaW50LmNvbS86eDovZy9wZXJzb25hbC92aTIxNjNzc19nb19taW5uc3RhdGVfZWR1L0VlNWFqNVc5cXRkRm4zNDRBQUtKTHVBQldtcEtWSUdNVmtJMkoxQzl3dmtPVlE_cnRpbWU9eEE2X291ZHIzVWc&CID=e2126631-9922-4cc5-b5d3-54c7007a756f&_SRM=0:G:93 Determine the amount of VRK1 is present 1. Average the data and calculate the mean absorbance for each concentration/dilution (Please over look for Corrections) 2. Blank Correction à Subtract 0 ug/mL blank absorbance from all readings (Please over look for Corrections) 3. Plot the Standard Curve (Please over look for Corrections) 4. Convert VRK1 concentration from ug/mL to g/L 5. Use the molar mass of VRK1 to convert to M and uM…

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Question

Chapter 16, Problem 4P

Interpretation Introduction

To Explain:

The state of rigor should be explained in molecular terms.

Introduction:

In muscle contraction, myosin filaments are being slide along the actin filaments. According to Albert Szent’s experiments, it is proven that the ATP hydrolysis and association and dissociation of actin and myosin complex are coupled. Energy released by ATP hydrolysis is used to power the muscle contraction.

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Please help determine the standard curve for my Kinase Activity in Excel Spreadsheet. Link: https://mnscu-my.sharepoint.com/personal/vi2163ss_go_minnstate_edu/_layouts/15/Doc.aspx?sourcedoc=%7B958f5aee-aabd-45d7-9f7e-380002892ee0%7D&action=default&slrid=9b178ea1-b025-8000-6e3f-1cbfb0aaef90&originalPath=aHR0cHM6Ly9tbnNjdS1teS5zaGFyZXBvaW50LmNvbS86eDovZy9wZXJzb25hbC92aTIxNjNzc19nb19taW5uc3RhdGVfZWR1L0VlNWFqNVc5cXRkRm4zNDRBQUtKTHVBQldtcEtWSUdNVmtJMkoxQzl3dmtPVlE_cnRpbWU9eEE2X291ZHIzVWc&CID=e2126631-9922-4cc5-b5d3-54c7007a756f&_SRM=0:G:93 Determine the amount of VRK1 is present 1. Average the data and calculate the mean absorbance for each concentration/dilution (Please over look for Corrections) 2. Blank Correction à Subtract 0 ug/mL blank absorbance from all readings (Please over look for Corrections) 3. Plot the Standard Curve (Please over look for Corrections) 4. Convert VRK1 concentration from ug/mL to g/L 5. Use the molar mass of VRK1 to convert to M and uM…

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