Chapter 16, Problem 41P

Summary Introduction

To determine:

The process by which RNA-Seq analysis can be used to find the components of Vibrio. fischeri quorum-sensing pathway and determining if any of the components of the pathway have escaped detection.

Introduction:

Vibrio. fischeri is a gram-negative bacteria having the ability of bioluminescence. These bacteria utilize quorum sensing to regulate the gene expression in response to changes in the cell density. RNA-sequencing is a technique used to study and analyze the expression of a full range of messenger RNA or transcriptome. It provides the ability to observe the undetected changes that occur in diseased states.