EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Textbook Question
Chapter 16, Problem 3MC
Rejection of a foreign skin graft is an example of ________________.
- a. destruction of virus-infected cells
- b. tolerance
- c. antibody-mediated immunity
- d. a secondary immune response
- e. a cell-mediated immune response
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 16 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 16 - Why are the activities of B and T cells called...Ch. 16 - Why are exogenous epitopes processed in vesicles...Ch. 16 - Why did scientists give the name perforin to a...Ch. 16 - Plasma cells are vital for protection against...Ch. 16 - Microsporidiosis Darius is sick, which is not...Ch. 16 - Why is passive immunity effective more quickly...Ch. 16 - Prob. 1MCCh. 16 - Prob. 2MCCh. 16 - Rejection of a foreign skin graft is an example of...Ch. 16 - An autoantigen is ________________. a. an antigen...
Ch. 16 - Among the key molecules that control cell-mediated...Ch. 16 - Which of the following lymphocytes predominates in...Ch. 16 - Prob. 7MCCh. 16 - Which cells express MHC class I molecules in a...Ch. 16 - In which of the following sites in the body can B...Ch. 16 - Tc cells recognize epitopes only when the latter...Ch. 16 - Prob. 1MTFCh. 16 - Prob. 2MTFCh. 16 - Prob. 3MTFCh. 16 - Prob. 4MTFCh. 16 - Prob. 5MTFCh. 16 - Match each cell in the left column with its...Ch. 16 - Match each type of immunity in the left column...Ch. 16 - Label the parts of the immunoglobulin below.Ch. 16 - The nearby image is a transmission electron...Ch. 16 - When is antigen processing an essential...Ch. 16 - Why does the body have both antibody and...Ch. 16 - Why is it advantageous for the lymphatic system to...Ch. 16 - Contrast innate defenses with adaptive immunity.Ch. 16 - How does requiring the immune system to process...Ch. 16 - Scientists can develop genetically deficient...Ch. 16 - Human immunodeficiency virus (HIV) preferentially...Ch. 16 - What would happen to a person who failed to make...Ch. 16 - Prob. 7CTCh. 16 - Some materials, such as metal bone pins and...Ch. 16 - Prob. 9CTCh. 16 - The cross-sectional area of the afferent lymphatic...Ch. 16 - Two students are studying for an exam on the bodys...Ch. 16 - In general, what sorts of pathogens might be able...Ch. 16 - What sorts of pathogens could successfully attack...Ch. 16 - As part of the treatment for some cancers,...Ch. 16 - Prob. 1CM
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