Nester's Microbiology: A Human Perspective
Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 16, Problem 2A
Summary Introduction

To review:

The effect of molecules interfering with the type III secretion system(T3SS) on pathogens.

Introduction:

T3SS is a protein appendage thatis present in most of the Gram-negative bacteria. In the pathogenic bacteria, a sensory probe, which is needle-like in the structure, is used to secrete the proteins that help in developing the infection in the eukaryotic cells. These secreted proteins are known as effector proteins and are secreted directly into the host cell.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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