Function of switch 1 of active model for K1F1A kinesin protein should be identified. Why K1F1A cannot move cargo when switch 1 is bound to AMPPNP should be explained. Introduction: A kinesin is a protein which acts as molecular motor in eukaryotic cells. Using the free energy by ATP hydrolysis kinesins move along microtubules. The active movement of kinesins involve in several cellular functions such as mitosis, meiosis and axonal transport. K1F1A is a member of kinesin family. A non-hydrolysable analogue of ATP is AMP-PNP. It enhances the interaction between membranous organelles and microtubules and inhibits axonal transport.

Question

Please draw out the mechanism with curved arrows showing electron flow. Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvate dehydrogenase complex, resulting in acetyl-CoA and CO2. Provide the mechanism for this reaction utilizing the TPP cofactor. Include the roles of all cofactors.

Answer 1

Question

Chapter 16, Problem 22P

Interpretation Introduction

To Explain:

Function of switch 1 of active model for K1F1A kinesin protein should be identified. Why K1F1A cannot move cargo when switch 1 is bound to AMPPNP should be explained.

Introduction:

A kinesin is a protein which acts as molecular motor in eukaryotic cells. Using the free energy by ATP hydrolysis kinesins move along microtubules. The active movement of kinesins involve in several cellular functions such as mitosis, meiosis and axonal transport. K1F1A is a member of kinesin family.

A non-hydrolysable analogue of ATP is AMP-PNP. It enhances the interaction between membranous organelles and microtubules and inhibits axonal transport.

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Please draw out the mechanism with curved arrows showing electron flow. Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvate dehydrogenase complex, resulting in acetyl-CoA and CO2. Provide the mechanism for this reaction utilizing the TPP cofactor. Include the roles of all cofactors.

Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvate dehydrogenase complex, resulting in acetyl-CoA and CO2. Provide the mechanism for this reaction utilizing the TPP cofactor. Include the roles of all cofactors.

The mitochondrial ATP synthase has 10 copies of the F0 subunit “c”, and the [H ] in the mitochondrial inner membrane space (IMS) is 6.31 x 10-8 M and the [H + ] in the matrix is 3.16 x 10-9 M. Calculate the minimum membrane potential (∆Ψ) necessary to make ATP synthesis thermodynamically favorable. [Assume ∆G' ofphosphate hydrolysis of ATP is - 45 kJ/mol.]