Concept explainers
To explain: The substance that make Mycobacterium resistant to staining.
Introduction: Bacteria is the unicellular prokaryotic organism that maintains a definite shape since it contains a rigid cell wall. It is classified into different types based on the structure, namely coccus (spherical shaped), Bacillus (rod-shaped), filamentous, spirilla (spiral-shaped), appendaged, and pleomorphic bacteria. The structure of the bacteria can be viewed under the microscope using staining methods.

Explanation of Solution
The bacteria such as Mycobacterium have cell walls with a high lipid content such as waxy material called mycolic acid. It is a complex lipid that is composed of fatty acids and fatty alcohols that contain hydrocarbon chains upto 80 carbons in length. This compound affects the staining properties of Mycobacterium and prevents it from being stained by many stains used in
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Chapter 16 Solutions
BENSON'S MICROBIOLOGY LAB CONCISE, LL+AC
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