2 SEM CARDLESS ACC W/RAVEN TEXT
2 SEM CARDLESS ACC W/RAVEN TEXT
12th Edition
ISBN: 9781265810467
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 16, Problem 1DA
Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Expert Solution
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Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution
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Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

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Students have asked these similar questions
What is the effect of an increased Cro concentration on the expression of the gene for the λ repressor? Of an increased concentration of λ repressor on the expression of the Cro gene? Of an increased concentration of λ repressor on the expression of the λ repressor gene?
Which of the following is the most likely result of a mutation in the lac operator that prevents release of the depressor protein? Choose 1 answer: (A)The lactose permease protein will be produced, even in the absence of lactose. (B) Transcription of the structural genes will be blocked, even in the presence of lactose. (C) The catabolite activator protein will be bound to the CAP site, even in the presence of glucose. (D) Expression of the lacI gene will be repressed, even in the absence of glucose.
What are the functions of transcriptional activator proteins and repressor proteins? Explain how they work at the molecular level.

Chapter 16 Solutions

2 SEM CARDLESS ACC W/RAVEN TEXT

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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY