Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 16, Problem 16.4PS
QUANTITATIVE DNA Melting. Figure 16-36 shows the melting curves for two DNA samples that were thermally denatured under the same conditions.
Figure 16-36 Thermal Denaturation of Two DNA Samples. See Problem 16-4.
- (a) What conclusion can you draw concerning the base compositions of the two samples? Explain.
- (b) How might you explain the steeper slope of the melting curve for sample A?
- (c) Formamide and urea are agents known to form hydrogen bonds with pyrimidines and purines. What effect, if any, would the inclusion of a small amount of formamide or urea in the incubation mixture have on the melting curves?
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Chapter 16 Solutions
Becker's World of the Cell (9th Edition)
Ch. 16 - Based on what you know about protein and DNA, why...Ch. 16 - The GC content of the DNA from a newly discovered...Ch. 16 - Prob. 1QCh. 16 - Changes in chromatin packing correlate with...Ch. 16 - You are studying a cytosolic protein, and your...Ch. 16 - Prior Knowledge. Virtually every experiment...Ch. 16 - DNA Base Composition. Based on your understanding...Ch. 16 - DNA Structure. Carefully inspect the...Ch. 16 - QUANTITATIVE DNA Melting. Figure 16-36 shows the...Ch. 16 - DNA Renaturation. You are given two samples of...
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