Concept explainers
(a)
Interpretation:
The length of the mirror derives in a spectrometer at resolution of
Concept introduction:
The FTIR-stands for the Fourier Transform Infrared Spectroscopy. Generally, this technique is used to determine the organic, inorganic and
The length of the mirror derives in a spectrometer is the half of the distance between the mirror derives. The distance between the mirror derives is reciprocal of the resolution of the FTIR spectrometer.
(b)
Interpretation:
The length of the mirror derives in a spectrometer at resolution of
Concept introduction:
The FTIR-stands for the Fourier Transform Infrared Spectroscopy. Generally, this technique is used to determine the organic, inorganic and polymeric materials.
The length of the mirror derives in a spectrometer is the half of the distance between the mirror derives. The distance between the mirror derives is reciprocal of the resolution of the FTIR spectrometer.
(c)
Interpretation:
The length of the mirror derives in a spectrometer at resolution of
Concept introduction:
The FTIR-stands for the Fourier Transform Infrared Spectroscopy. Generally, this technique is used to determine the organic, inorganic and polymeric materials.
The length of the mirror derives in a spectrometer is the half of the distance between the mirror derives. The distance between the mirror derives is reciprocal of the resolution of the FTIR spectrometer.
Trending nowThis is a popular solution!
Chapter 16 Solutions
Principles of Instrumental Analysis
- (b) An aqueous solution whose concentration is 0.00344 M was analysed using spectrophotometer with a molar absorptivity of 412 cm-1 mo1 L in a cell with a 2.00 cm pathlength. Calculate the absorbance and transmittance for the above solution.arrow_forwardAlexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.arrow_forwardExplain why an internal standard is required during sample preparation in quantitative analysis when using atomic spectroscopy?arrow_forward
- A Raman spectrum of undoped GaAs at room temperature is performed with a 514.5 nm laser. The spectrum of scattered light exhibits peaks at wavelengths of 507.1 nm and 591.6 nm due to interaction of the incident light with LO phonons. Calculate the LO phonon frequency and the ratio of the intensity of scattered light at 507.1 nm to that at 591.6 nm.arrow_forward(a) You measure the percent transmittance of a solution containing chromophore X at 400nm in a 1-cm path length cuvette and find it to be 50%. What is the absorbance of this solution? (b) What is the molar absorptivity of chromophore X if the concentration of X in the solution measured in question (a) is 0.5mM? (c) What is the concentration range of chromophore X that can be assayed if, when using a sample cell of path length 1, you are required to keep the aborbance between 0.2 and 0.8?arrow_forwardAssuming an integrated luminosity of 100 fb is collected at the ATLAS detector at the LHC, calculate the number of h ZZ events where both Z bosons decay to either a ete pair or a u u palr. You may assume that the total Higgs (h) production cross section is o, = 2 x 10' fb, the branching fraction for a Z boson decay to a ee pair is 3.36%, the branching fraction for a Z boson decay to a "u pair is 3.36%, and the branching fraction for a Higgs boson to decay to a pair of Z bosons is 2.7%. Enter your answer in the box below rounded to the nearest integer (e.g. 1234.4 should be entered as 1234). Ancwerarrow_forward
- If two signals differ by 90 Hz in a 300 MHz spectrometer, by how much do they differ in a 500 MHzspectrometer?arrow_forwardoutline the general procedure for determining a substance spectrophotometricallyarrow_forwardQ4.2 Sensitivity If 13C data were collected at this field using a protein concentration of 500 µM what is the effective protein concentration that gives rise to the measured NMR signal? Enter your answer herearrow_forward
- (a) Three components in a mixture have varying distribution constants between the mobile phase and the stationary phase. One of the components has a very high distribution constant. Would this component likely elute first or last and how would you design an experiment or modify the components of the separation to make it perform in the opposite manner? (b) One way to dramatically increase the sensitivity of a quadrupole mass spectrometer is to use a technique called selected ion monitoring (SIM). Explain how this technique works and why it improves sensitivity. Explain what dwell time means. Be sure to draw a diagram and describe how this analysis scheme works. Also, is there a compromise between a wide range of masses scanned and SIM? Explain in easy details.arrow_forwardIf the measured absorbance at 280 nm of the protein in a 1.00-cm cuvette = 0.564, what is the extinction coefficient at this wavelength?arrow_forwardGiven C6H12O2 chemical formula. chemical shift , intergration, multiplicity and interpretation needed.arrow_forward
Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning