BIOL 1010/1020 CLASS ONLY CONNECT
BIOL 1010/1020 CLASS ONLY CONNECT
14th Edition
ISBN: 9781260038644
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 15.4, Problem 3AQTC
Summary Introduction

To determine:

The view of smoking bans based on the fact that 90 million nonsmokers in United States have measurable levels of toxic chemicals in their body resulting from second hand smoke.

Introduction:

Smoking is addictive to people as nicotine is an addictive drug. It is known that smoking has been banned in many public spaces as more people are exposed to second-hand smoke. People in the vicinity of smokers are exposed to vapors of nicotine.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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