Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 15.2, Problem 4QC
What happens in an olfactory neuron when an odorant binds to its receptor?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 15 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 15.1 - What are the three major differences between the...Ch. 15.1 - What is sensory transduction?Ch. 15.2 - 1. Where is the olfactory epithelium located?
Ch. 15.2 - 2. What types of cells make up the olfactory...Ch. 15.2 - Describe the structure of an olfactory neuron.Ch. 15.2 - What happens in an olfactory neuron when an...Ch. 15.2 - How can humans detect such a variety of odors with...Ch. 15.2 - Prob. 6QCCh. 15.3 - Where are taste buds located?Ch. 15.3 - Prob. 2QC
Ch. 15.3 - Prob. 3QCCh. 15.3 - Prob. 4QCCh. 15.3 - How does a gustatory cell transduce a chemical...Ch. 15.3 - 6. Which cranial nerves transmit taste sensation...Ch. 15.3 - 7. Which part of the brain is responsible for...Ch. 15.4 - What are the functions of the tarsal glands and...Ch. 15.4 - Trace the path of tears as they travel from the...Ch. 15.4 - 3. Which two extrinsic eye muscles move the eye...Ch. 15.4 - Prob. 4QCCh. 15.4 - 5. What are the functions of each component of...Ch. 15.4 - 6. What are the two fluids found in the eyeball?...Ch. 15.5 - Prob. 1QCCh. 15.5 - Prob. 2QCCh. 15.5 - What is accommodation?Ch. 15.5 - Prob. 4QCCh. 15.5 - 5. What is the near point of accommodation, and...Ch. 15.5 - 6. How do myopia and hyperopia differ?
Ch. 15.5 - How are rods different from cones?Ch. 15.5 - Why are photoreceptors depolarized in the dark?Ch. 15.5 - How do rods hyperpolarize when light strikes them?...Ch. 15.5 - 10. What happens at the optic chiasma? What is...Ch. 15.5 - 11. What type of visual processing occurs in the...Ch. 15.6 - 1. What is the auricle?
Ch. 15.6 - What is the purpose of cerumen?Ch. 15.6 - 3. Where is the tympanic membrane located?
Ch. 15.6 - Prob. 4QCCh. 15.6 - What is the role of the pharyngotympanic tube?Ch. 15.6 - What are the functions of the utricle, saccule,...Ch. 15.6 - How are the scala tympani, scala media, and scala...Ch. 15.6 - 8. What is the spiral organ, and where is it...Ch. 15.7 - 1. What physical properties determine the pitch...Ch. 15.7 - 2. What is the range (in hertz) of human...Ch. 15.7 - 3. Why is the force exerted on the oval window...Ch. 15.7 - Prob. 4QCCh. 15.7 - Prob. 5QCCh. 15.7 - Prob. 6QCCh. 15.7 - Prob. 7QCCh. 15.7 - Prob. 8QCCh. 15.8 - How do static and dynamic equilibrium differ?Ch. 15.8 - 2. What role do the utricle and saccule play in...Ch. 15.8 - What role do they play in dynamic equilibrium?Ch. 15.8 - Prob. 4QCCh. 15.8 - 5. What are the three primary areas of the...Ch. 15 - Match the cell type with the correct stimulus....Ch. 15 - 2. The axons of the olfactory nerve terminate in...Ch. 15 - 3. Fill in the blanks: In an olfactory neuron, the...Ch. 15 - The primary olfactory cortex is located in the: a....Ch. 15 - 5. Which of the following statements is true...Ch. 15 - Match the taste with the chemical substance that...Ch. 15 - Prob. 7CYRCh. 15 - Prob. 8CYRCh. 15 - 9. Which cells in the retina are depolarized in...Ch. 15 - Prob. 10CYRCh. 15 - Each of the following statements is false. Correct...Ch. 15 - The axons from the nasal retina in the left eye...Ch. 15 - Mark the following statements as true or false. If...Ch. 15 - Explain how sounds of different frequencies are...Ch. 15 - Prob. 15CYRCh. 15 - Prob. 16CYRCh. 15 - Prob. 17CYRCh. 15 - 18. True or false: Hair cells in the spiral organ...Ch. 15 - 19. Which of the following is not part of the...Ch. 15 - 20. Fill in the blanks: In the ampulla of a...Ch. 15 - Stimuli from the inner ear regarding head movement...Ch. 15 - 1. Explain what would happen to your sense of...Ch. 15 - In which direction would you be unable to move...Ch. 15 - If a patient suffers visual impairment only in one...Ch. 15 - 4. Following a stroke, a patient lost vision in...Ch. 15 - Why do you have the sensation that you are still...Ch. 15 - 1. Mr. Spencer suffers loss of taste sensation....Ch. 15 - 2. Your friend tells you that she has just been...Ch. 15 - Prob. 3AYKACh. 15 - Prob. 4AYKACh. 15 - Mrs. Flores is a 45-year-old female who suffered a...Ch. 15 - 6. Your 60-year-old patient, Mr. Guster, has...
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